Recombinant Human HMGB1/High Mobility Group Protein B1/HMG-1 #abs04694

abs04694-10ug

1-2 Weeks

161

abs04694-50ug

1-2 Weeks

482

Description

Our E.coli expression system is responsible for producing Recombinant Human High Mobility Group Protein B1. This system effectively expresses the target gene encoding Gly2-Phe89. To put it differently, we utilize our E.coli expression system to generate Recombinant Human High Mobility Group Protein B1, which is primarily composed of the amino acids Gly2-Phe89.

Other names

HMGB1, also known as High Mobility Group Protein 1 or High Mobility Group Protein B1, is a protein that plays an important role in various cellular processes. This important protein has been extensively researched due to its involvement in numerous physiological and pathological conditions.
HMGB1 is a DNA-binding protein that is involved in the regulation of gene expression, DNA repair, and genome stability. Additionally, it functions as an extracellular signaling molecule that plays a role in inflammation, immunity, and tissue repair.
The release of HMGB1 into the extracellular environment can occur during cellular stress or damage and can trigger various cellular responses. It has been implicated in various diseases, including cancer, sepsis, and autoimmune disorders.
Overall, the study of HMGB1 continues to shed light on its complex and multifaceted properties, making it an area of active research in the field of cell biology and disease pathology.

Source

E.coli

Format

The solution was filtered through a 0.2 μm filter and then lyophilized to form a powder. The powder was derived from a mixture of 20mM PB and 150mM NaCl at a pH of 7.4.

Properties

Concentration

SDS-PAGE，95％。，，。

Stability & Storage

To preserve the integrity of lyophilized protein, it is recommended to store it at below -20°C. However, stabilized protein can remain at room temperature for up to 3 weeks. After reconstitution, the protein solution should be kept at a temperature between 4-7°C for 2-7 days. Lengthy storage is not advised, and aliquots of reconstituted samples should be stored at below -20°C for a maximum of 3 months.

Target

Background

HMGB1, a member of the HMGB family, is a protein known for its high mobility. This family includes three members, HMGB1, HMGB2, and HMGB3. HMGB1 has two HMG box DNA-binding domains called box A and box B. At the C terminus, it possesses a highly negative-charged region. Functioning as a nuclear protein, HMGB1 plays a crucial role in stabilizing nucleosomes and facilitating the bending of DNA, thereby promoting gene transcription necessary for the survival of individuals. However, recent findings have unveiled another role of HMGB1 as an extracellular cytokine. It exerts regulatory effects on monocytes, T cells, and dendritic cells during inflammatory responses. This dual role highlights the versatile nature of HMGB1 and its significance in both intracellular and extracellular processes.