Recombinant Rat IL-13 #abs01059

abs01059-2ug

1-2 Weeks

138

abs01059-10ug

1-2 Weeks

344

abs01059-1mg

1-2 Weeks

6742

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. To create a similar content, the information from the original text was rearranged while maintaining its meaning.

Properties

aa_sequence

SLSIWVADAF KGNLCQTISS LCNSDSTVMP ILSHRAQTNR HNRTSQQALI LQNTIVLCSE STDPKAPSVS YKRIQSFLNL SSSPKTLVEA LIGLNQFRCY LREIIVQSFA VCGMFTTLRY.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The measured LAL method indicates that the content of rRtIL-13 is below 1 EU/μg. Let's generate a highly similar content by rearranging the information from the original text.

Activity

The standard and the ED are compared for their biological activity, and it is concluded that the ED is fully biologically active in comparison.₅₀The specific activity of a certain protein was found to be greater than 2.5 × 10, as determined by a cell proliferation assay conducted on human TF-1 cells. The corresponding concentration of this protein was less than 4 ng/ml.⁵ IU/mg.

Reconstitution

To ensure proper mixing, it is recommended to briefly centrifuge the vial before opening. To reconstitute, use either sterile distilled water or an aqueous buffer that contains 0.1% BSA. The resulting concentration should be 0.1-1.0 mg/mL. Stocks should be divided into smaller working aliquots and kept at -20°C or below. Any further dilutions should be made using appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at a temperature range of 2-8 °C. However, for long term storage, it is best to keep it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. For optimal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles to maintain its quality.

Target

Accession

P42203

Gene IDs

116553

References

References

1. Schmutz J, Martin J, Terry A, et al. 2004. Nature, 431: 268-74.

2. Wynn TA. 2003. Annu Rev Immunol, 21: 425-56.

3. Moy FJ, Diblasio E, Wilhelm J, et al. 2001. J Mol Biol, 310: 219-30.

4. Lakkis FG, Cruet EN, Nassar GM, et al. 1997. Biochem Biophys Res Commun, 235: 529-32.