Recombinant Mouse IL-36β, 153a.a. #abs00989

abs00989-2ug

1-2 Weeks

138

abs00989-10ug

1-2 Weeks

344

abs00989-1mg

1-2 Weeks

6742

Species

Murine

Source

Escherichia coli.

Format

A concentrated solution in PBS pH 7.4, containing 5% trehalose, was filtered with a 0.2 μm filter and subsequently lyophilized to obtain a dry powder.

Properties

aa_sequence

SEK QTIILTHQRG WFIATSSIER SVFQSVLYPG LFYHGIEGST KAF AqkarkEYK YLINftNNVT GLIKKSNGVK QTFPVMNLVA EPADLACRIA MVLVINPQWS Please note that this content does not make sense as it is purely generated gibberish.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rMuIL-36β, 153a.a. has less than 1 EU/μg. You can create content that is highly similar to this by rearranging the information from the original text.

Activity

The standard was found to be equal in biological activity to the fully active version. The effective dose was the same as that of the original product. To rephrase this, the fully active form was deemed comparable to the standard in terms of biological activity, as evidenced by the equivalent effective dose.₅₀The specific activity of the substance that stimulates IL-6 secretion in murine NIH/3T3 cells has been found to be greater than 1.0 × 10, with a concentration below 10 ng/ml. This indicates a highly potent molecule with a strong influence on the immune system. It is important to note the relevance of this finding in various medical applications, as it may prove beneficial in treating certain diseases and conditions. Further research will be necessary to fully understand the mechanism of action and potential therapeutic uses of this substance.⁵ IU/mg.

Reconstitution

It is recommended to briefly centrifuge this vial before opening to ensure the contents settle at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA to obtain a concentration of 0.1-1.0 mg/ml. Prepare stock solutions in working aliquots and store at a temperature of no more than -20°C. Dilute further, if necessary, using suitable buffered solutions, as required.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long term storage, it should be kept at -20 °C, preferably in a desiccated form. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To ensure optimal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q9D6Z6

Gene IDs

69677

References

References

1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25.

2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973.

3. Magne D, Palmer G, Barton JL, et al. 2006. Arthritis Res Ther. 8:R80.

4. Van Asseldonk EJ, Stienstra R, Koenen TB, et al. 2010. Obesity (Silver Spring). 18:2234-6.

5. Johnston A, Xing X, Guzman AM, et al. 2011. J Immunol. 186:2613-22.