Recombinant Human IL-36RA #abs00836

abs00836-5ug

1-2 Weeks

138

abs00836-25ug

1-2 Weeks

344

abs00836-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, was filtered through a 0.2 μm filter and then lyophilized. To create content that is highly similar to the original text, this information can be rearranged and stated as follows: The solution was first concentrated and filtered through a 0.2 μm filter before being lyophilized. This was done in PBS with a pH of 7.4.

Properties

aa_sequence

QLSGLCVFAR MKSLDKLVLY NHGLAQGGLL VKHAGGKEVI EPNSVLRWDP SILSVILPGV GQGSCCLQCG TLEPVTGNQE YLMINELKGA FTYKSFSERS GLTSSFDGRM PGWFAAYEPS TQPVDEAPCV GGENLPQTWL FDFYPITAWN QCDCQFQFQQ VVAVLLSGAC FMRKDALKVYL HNNAQLLAGL LHAGKIVGEE ISSVPRWLDL ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of Recombinant Human IL-36RA detected by the LAL method is lower than 1 EU/μg. Let me rephrase this information in a different way. The LAL method has determined that the Recombinant Human IL-36RA has a concentration of less than 1 EU/μg.

Activity

Compared to the standard, the ED exhibits full bioactivity. To create a closely resembling content, the following text can be considered: The ED is demonstrated to possess complete biological activity in comparison to the standard.₅₀The specific activity of inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is greater than 1000 IU/mg when the concentration is less than 1.0 μg/ml. This activity was observed in the presence of 20 ng/ml of recombinant human IL-36 beta.

Reconstitution

To ensure proper mixing of the contents, it is recommended to briefly centrifuge the vial before opening. The powder can then be reconstituted in sterile distilled water or an aqueous buffer containing 0.1% BSA, with a desired concentration of 0.1-1.0 mg/mL. It is recommended to divide the stock solution into smaller working aliquots and store at -20°C or lower. Should further dilutions be required, it is important to use appropriate buffered solutions for accurate results.

Stability & Storage

To maintain the integrity of this lyophilized preparation, it is recommended to store it at 2-8°C. However, for long-term storage, it is best to keep it at -20°C, preferably with desiccation. When reconstituted, the preparation can remain stable for up to one week at 2-8°C. To ensure the maximum longevity of the product, it is advised to divide the reconstituted preparation into working aliquots and freeze them at -20°C to -70°C. Avoid multiple freezing and thawing cycles, as this can compromise the stability and effectiveness of the preparation.

Target

Accession

Q9UBH0

Gene IDs

26525

References

References

1. Born TL, Smith DE, Garka KE, et al. 2000. J Biol Chem. 275:41528.

2. Dunn EF, Gay NJ, Bristow AF, et al. 2003. Biochemistry. 42:10938-44.

3. Barton JL, Herbst R, Bosisio D, et al. 2000. Eur J Immunol. 30:3299-308.

4. Debets R, Timans JC, Homey B, et al. 2001. J Immunol. 167:1440-6.

5. Towne JE, Renshaw BR, Douangpanya J, et al. 2011. J Biol Chem. 286:42594-602.