Recombinant Equine IL-2 Cys141Ser #abs01101

abs01101-2ug

1-2 Weeks

138

abs01101-10ug

1-2 Weeks

344

abs01101-1mg

1-2 Weeks

5058

Species

Equine

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 6.5, was filtered through a 0.2 μm filter and subsequently lyophilized to produce a dry powder. This process ensures that the resulting product is highly pure and stable, making it ideal for various applications. The lyophilization process involves removing the water from the solution by freezing it and then subjecting the frozen mixture to a vacuum, thus creating a dry powder. This method is particularly useful for preserving biological materials such as proteins and enzymes. The resulting product can be easily reconstituted with water to form a solution that is ready for use.

Properties

aa_sequence

FSSKMSPTNK TNKVNNLEGL LPKMLELKQL DMDLQKQLQQ ERKITFKNMP ELMKKLELHQ CLEQHLKELT KEDKISLFLN VLTVNINMSL FRTEGSKGGL FTITGWKNVE ITGTDDEYCE MTSFILNKWE MTSTFSIQF.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rEqIL-2 Cys141Ser has a level of less than 0.1 EU/μg. This indicates a highly minimal presence of endotoxins in the substance.

Activity

The biological activity of the sample is equivalent to that of the standard, indicating full biologic functionality. The Effective Dose (ED) of the sample is also on par with the standard.₅₀The specific activity of the substance, which was assessed through a cell proliferation assay involving murine CTLL-2 cells, is greater than 1000 IU/mg, with a concentration of less than 1.0 μg/ml. This indicates that the substance has a potent effect on cell growth and division.

Reconstitution

To ensure optimal results, we advise that the vial is subjected to brief centrifugation before opening, in order to sediment the contents. Subsequently, the contents should be reconstituted in either sterile distilled water or an aqueous buffer containing 0.1 % BSA, at a concentration ranging from 0.1-1.0 mg/mL. Stock solutions should be divided into workable aliquots, and stored at or below -20 °C. Dilutions should be performed using suitable buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized product, it should be stored at 2-8 °C, though long-term storage is recommended at -20 °C, preferably with desiccation. Once reconstituted, the preparation can be stored for up to a week at 2-8 °C. However, for the utmost stability, divide the reconstituted solution into working aliquots and store them at -20 °C to -70 °C. Avoid the risk of repeated freeze/thaw cycles.

Target

Accession

P37997

Gene IDs

100034204