Recombinant Human IFN-α2b, Yeast #abs00915

abs00915-20ug

1-2 Weeks

138

abs00915-100ug

1-2 Weeks

344

abs00915-1mg

1-2 Weeks

1345

Source

Yeast

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. The reconstitution buffer used was PBS, pH 7.4, with 0.02 % Tween-20. Kindly note that this content is different from the original text information as it has been generated using a language model.

Properties

aa_sequence

LDKFYTELYQ CDLPQTHSLG QQIFNLFSTK MRRISLFSCL SRRTLMLLAQ KDRHDFGFPQ DSSAAWDETL LRSKE FSLSTNLQES EVVRAEIMRS IKRGDRPGMM QLNDLEACVI KEDSILAVRR KYFQRITLYL KEKKYSPCAW EEFGNQFQKA ETIPVLHEMI QGVGVTETPL
The sequence of letters CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE can be rearranged in multiple ways to form new sequences. Here's one possible sequence that maintains the integrity of the original letters:
LDKFYTELYQ CDLPQTHSLG QQIFNLFSTK MRRISLFSCL SRRTLMLLAQ KDRHDFGFPQ DSSAAWDETL LRSKE FSLSTNLQES EVVRAEIMRS IKRGDRPGMM QLNDLEACVI KEDSILAVRR KYFQRITLYL KEKKYSPCAW EEFGNQFQKA ETIPVLHEMI QGVGVTETPL
While the sequence may appear to be random, it's important to note that each letter remains unchanged and the original message is still encoded in the rearranged sequence.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determined LAL method confirms that the Recombinant HumanIFN-α2b, Yeast contains less than 0.1 EU/μg. Let's rearrange the wording to create a similar content: "According to the LAL method, the Recombinant HumanIFN-α2b, Yeast has been found to possess an EU/μg value of less than 0.1, indicating its high quality and purity."

Activity

The standard and our product exhibit equal biological activity, as confirmed by a comparison analysis. Furthermore, we conducted an anti-viral assay, and the specific activity of our product was determined to be no lower than 1.6 × 10, implying its potency and efficacy.⁸ IU/mg.

Reconstitution

To ensure proper mixing, it is recommended to briefly centrifuge the vial before opening. Reconstitute the contents in sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. It is advisable to divide the stock solution into separate working aliquots and store them at temperatures equal to or below -20 °C. For further dilutions, make use of appropriate buffered solutions.

Stability & Storage

To ensure optimal stability, it is recommended to store this lyophilized preparation at -20 °C for long term storage, after first maintaining it at 2-8 °C. A desiccated environment is preferred. After reconstitution, refrigeration at 2-8 °C is recommended for use within one week. For prolonged stability, divide the reconstituted preparation into working aliquots and store at -20 °C to -70 °C, taking care to avoid repeated freeze/thaw cycles.

Target

Accession

P01563

Gene IDs

3440

References

References

1. Tarhini AA, Gogas H, Kirkwood JM. 2012. J Immunol, 189: 3789-93.

2. Tohyama M, Yang L, Hanakawa Y, et al. 2012. J Invest Dermatol, 132: 1933-5.

3. Corssmit EP, Heijligenberg R, Hack CE, et al. 1997. Clin Exp Immunol, 107: 359-63.

4. Corssmit EP, de Metz J, Sauerwein HP, et al. 2000. J Interferon Cytokine Res, 20: 1039-47.