Recombinant Mouse MIP-1γ/CCL9/CCL10 #abs01175

abs01175-5ug

1-2 Weeks

138

abs01175-20ug

1-2 Weeks

344

abs01175-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2μm filter before being lyophilized. From this lyophilized sample, a highly similar content can be generated by rearranging the previous sentence. Essentially, the original sample was concentrated in PBS with a pH of 7.4 before being filtered and dried using lyophilization.

Properties

aa_sequence

GYFPTSGGCT VQSSLKAQQG RPGIIFISKR QDSSDCCLSY QITHATETKE NSRIQCSRFI LEIEMFHMGF RRVQRCIERL GFQVCANPSD EQNSQPRTYK

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the amount of rMuMIP-1γ/CCL9/CCL10 is below 1 EU/μg. Let me generate a similar content by rearranging the original information.

Activity

The standard and the sample are completely biologically active, and the human neutrophil chemotaxis bioassay confirms this. The concentration range of the bioactivity is 0.1-10 ng/ml, which is a strong indication of the potency of the sample.

Reconstitution

To ensure optimal results, it is recommended to briefly centrifuge the vial before opening to bring the contents to the bottom. The reconstitution should be done using sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Any remaining stock solutions should be stored at -20°C or lower with working aliquots to avoid multiple freeze-thaw cycles. Further dilutions should be made using appropriate buffered solutions to maintain the integrity of the protein.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it should be kept at a much colder temperature of -20 °C, preferably in a desiccated condition. Once reconstituted, the preparation can be stored in a refrigerator for up to a week. To ensure maximum stability, divide the reconstituted preparation into smaller aliquots and store them between -20 °C to -70 °C. To prevent damage to the preparation, avoid exposing it to repeated freeze and thaw cycles.

Target

Accession

P51670

Gene IDs

20308

References

References

1. Youn BS, Jang IK, Broxmeyer HE, et al. 1995. J Immunol. 155:2661-7.

2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51.

3. Lean JM, Murphy C, Fuller K, et al. 2002. J Cell Biochem. 87:386-93.