Insulin Transferrin Selenium Sodium Pyruvate Additive（ITS-A),100X

The use of the insulin-transferrin-selenium additive allows for a decrease in the FBS concentration needed during the routine maintenance and low-density adherent culture of various cell types. By incorporating this additive, researchers can achieve the necessary cell growth and development with less reliance on high FBS concentrations. This finding showcases the potential benefits of utilizing insulin-transferrin-selenium as a supplement in cell culture experiments, reducing costs and FBS usage without compromising the quality of cell growth.To prepare the Earle's Balanced Salt Solution (EBSS) according to ITS (Insulin-Transferrin-Selenium) Earle's equilibrium salt solution, follow these steps:
1. Gather the required ingredients for the EBSS preparation.
2. Ensure you have the necessary equipment such as a sterile container, a magnetic stirrer, and a pH meter.
3. Begin by measuring distilled water to meet the desired volume of the EBSS solution. It should be prepared in a sterile environment to prevent any contamination.
4. Transfer the measured distilled water into the sterile container.
5. Add the individual salts and components in the following order: sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), magnesium chloride (MgCl2), sodium bicarbonate (NaHCO3), sodium phosphate dibasic (Na2HPO4), and glucose.
6. Use a magnetic stirrer to mix the solution thoroughly until all the components are dissolved completely.
7. Check the pH of the solution using a pH meter. Adjust the pH if necessary by adding small amounts of hydrochloric acid (HCl) or sodium hydroxide (NaOH) while monitoring the pH level until it reaches the desired range (usually between pH 7.2 to 7.4).
8. Once the pH is within the desired range, check the overall volume of the solution again. Adjust it if needed to meet the desired final volume.
9. Sterilize the EBSS solution using an appropriate method such as autoclaving or filtration, depending on the requirements of your experiment.
10. Store the prepared EBSS solution in a sterile container at the recommended temperature until ready for use in your experiment.
By following these steps, you can successfully prepare the ITS Earle's equilibrium salt solution (EBSS) for various research or experimental purposes.There are three different types of ITS provided by Source Culture: ITS-G (ABS9462) for general use, ITS-A (ABS9463) for adherent cell culture, and ITS-X (ABS9464) specifically designed for use in composite media that contain sodium pyruvate, such as F-12 or DMEM/F-12. These ITS variants cater to specific requirements and ensure optimal cell growth and development in various culture conditions. With ITS-G, researchers can rely on a versatile and reliable ITS product for their general cell culture needs. ITS-A is designed to enhance cell adhesion, making it suitable for adherent cell culture applications. Lastly, ITS-X is tailored for composite media containing sodium pyruvate, enabling researchers to successfully cultivate cells in such environments. Source Culture's range of ITS offerings allows researchers to choose the most suitable option based on their specific culture requirements.，2%~4%（FBS），FBS。，。

Water-for-injection is the main ingredient used in the preparation of this product. To ensure top quality, it is carefully formulated using this pure and sterile water.

Our product serves the purpose of facilitating scientific research and production activities, specifically designed for the in vitro cultivation of tissues and cells. It caters to both research and production needs, providing a reliable platform for the culture of tissues and cells in laboratory settings. Clinical use is prohibited.