Human IL-29 High Sensitivity ELISA Kit

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Testing Principle：

This particular kit utilizes a double antibody sandwich ELISA approach. The high affinity conjugate plate comes prepackaged with a specific anti-human CXCL13 Capture Antibody. In order to perform the test, the standard substance, sample to be tested, and a biotin labeled detection antibody are sequentially added into the HRP-plate well. Following thorough mixing, the CXCL13 present in the sample undergoes a 2-hour incubation process at room temperature. During this time, the CXCL13 combines with the solid phase antibody and detection antibody, effectively forming a sandwich. Next, a horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added to the well. After another round of washing, TMB color substrate is added and incubated for color development in darkness at room temperature. The final depth of the color reaction will be positively correlated with the concentration of CXCL13 in the sample. Lastly, a termination solution is added and the absorbance value is measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：We provide a comprehensive kit that includes a pre-coated 96-well plate, allowing for easy and efficient testing. The kit also contains standards to ensure accurate results. Additionally, it includes a CXCL13 test antibody, standard diluent, and test buffer for optimal performance. To generate reliable and consistent results, we have included TMB color substrate, washing solution, and stop solution. The kit also includes SA-HRP for enhanced detection. For convenience, we provide a seal plate film to protect the samples. Detailed instructions are provided to guide you through the testing process.

Sensitivity：0.08 pg/mL

Detection range：3.13-200 pg/mL

Recovery rate： 86-108%

Storage：2-8℃

The standard curve：

Background：

IL-29, also known as interferon lambda-1, is a helix cytokine and is a type III interferon. It shares a high degree of sequence similarity with another type III interferon, IL-28. Interestingly, while the IL-29 gene is present in the human chromosome 19, it is absent in the mouse genome. This cytokine is crucial in defending the host from microorganisms. Its gene is upregulated significantly in virus-infected cells, and it possesses substantial anti-viral activity and immunomodulatory properties. IL-29 can inhibit viral replication by inducing a cellular antiviral response and activating similar pathways as IFN-α/β. However, IL-29 binds to a unique receptor, allowing it to work together with other cytokines to inhibit viral replication during natural and chronic viral infections such as hepatitis C. The antiviral activity of IL-29 is achieved by regulating proteins and molecules involved in immune responses. When the ligand/receptor complex forms, it signals through the JAK-STAT pathway. Overall, IL-29 is a vital component of the immune system and plays a crucial role in host defense against infections.

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