Sucrose Phosphate Synthase Microplate Assay Kit

Sucrose-phosphate synthase (SPS), also known as sucrosephosphate-UDP glucosyltransferase or UDP-glucose-fructose-phosphate glucosyltransferase, is an enzyme encoded by the EC 2.4.1.14 gene. This enzyme plays a crucial role in the synthesis of sucrose-phosphate from UDP-glucose and fructose-phosphate. SPS serves as a key regulatory enzyme in the process of sucrose metabolism and plays a significant role in plant growth and development.
The importance of SPS lies in its ability to catalyze the transfer of a glucose moiety from UDP-glucose to fructose-phosphate, leading to the formation of sucrose-phosphate. This process is essential for the efficient conversion of photosynthetic carbon into sucrose, a major carbohydrate in plants.
SPS is found in various plant tissues, including leaves, stems, and fruits. Its expression and activity levels are regulated by various factors, such as light, hormonal signals, and metabolic status. Furthermore, SPS activity can be modulated by phosphorylation, which affects its catalytic efficiency.
The function of SPS in plants goes beyond sucrose synthesis. It also participates in the regulation of carbon partitioning and acts as a signaling molecule in response to environmental cues. SPS has been linked to various physiological processes, including seed development, flowering, and stress responses.
In summary, sucrose-phosphate synthase is a vital enzyme involved in sucrose metabolism in plants. Its multifaceted roles make it a fascinating subject for further research in understanding plant physiology and developing strategies to enhance crop yield and stress tolerance.

Sucrose Phosphate Synthase Activity can be detected and quantified using various methods and techniques. These methods are based on the principle of measuring the production of sucrose phosphate or other metabolites that are produced as a result of the enzyme's activity.
One method that is commonly used for detection and quantification of Sucrose Phosphate Synthase Activity is the spectrophotometric assay. This assay measures the change in absorbance of the reaction mixture at a specific wavelength, which is directly proportional to the enzyme activity.
Another method that can be used is the radioactive assay, which utilizes radiolabeled substrates to measure the enzyme's activity. This method is highly sensitive and can detect even low levels of enzyme activity.
Overall, the detection and quantification of Sucrose Phosphate Synthase Activity is essential for understanding the role of this enzyme in plant metabolism and can help in the development of new strategies for crop improvement.

100 ug/ml - 4000 ug/ml

Shipped and store at 4°C for 6 months.