Six Color Multiplex Fluorescent Immunostaining Kit (Anti Rabbit)

In the tissue microenvironment, there exists a multitude of intricate cells. The characteristics of these cells, including their state, quantity, distribution and phenotype, are crucial to both biological significance and clinical significance. It can be shown in situ by antibody staining.Immunohistochemical staining is a prevalent technique for investigating the morphology of tissues and the expression of proteins in situ. However, conventional IHC techniques only permit the detection of a single indicator, which makes it challenging to identify the composition, state, and intercellular relationships in the complex tissue microenvironment. This information is critical for the accurate diagnosis and treatment of various diseases. Therefore, advanced techniques that allow for a more comprehensive analysis of tissue microenvironments are essential in modern medicine.

The tyrammonia signal amplification technology operates on a similar principle as the DAB color rendering method in traditional immunohistochemistry. Here, the technique also utilizes a secondary antibody labeled with HRP. The HRP enzyme then facilitates the addition of a fluorescepin substrate to the system, resulting in the generation of an activated fluorescent substrate. This activated substrate possesses the ability to covalently bind with tyrosine residues present on the antigen. Consequently, a stable covalent bond is formed, enabling the attachment of fluorescepin to the sample. By rearranging the given information, a similarly informative content can be generated.Following the initial step, the antibodies that were bound through non-covalent interactions underwent a hot repair process to remove them. Subsequently, a second incubation was carried out with another primary antibody and a different luciferase substrate. This step was essential to achieve multiple labeling of the target.