Phosphofructokinase Microplate Assay Kit

The enzyme with the EC number 2.7.1.11 is commonly known as PFK or 6-phosphofructokinase. It is also referred to as Phosphofructokinase I or Phosphohexokinase.

Phosphofructokinase activity can be detected and quantified using specific methods. These techniques aim to measure the activity level of phosphofructokinase, an important enzyme involved in cellular metabolism. By determining the level of enzyme activity, researchers can gain valuable insights into metabolic processes. One approach is to design an assay that directly measures the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, which is catalyzed by phosphofructokinase. This reaction can be coupled with another enzymatic step to produce a measurable signal, such as a change in absorbance or fluorescence. By monitoring the signal over time, it is possible to quantify the activity of phosphofructokinase in the sample. Various techniques, such as spectrophotometry and fluorometry, can be used to detect and measure the signal accurately. Furthermore, it is essential to establish proper controls and calibration standards to ensure the accuracy and reliability of the assay. Overall, the detection and quantification of phosphofructokinase activity provide valuable information about cellular metabolism and can contribute to a better understanding of metabolic disorders and diseases.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.