Alanine Transaminase Microplate Assay Kit

The enzyme EC 2.6.1.2, also known as alanine transaminase or ALT, is an important component of liver function tests. ALT is involved in the transfer of an amino group between the amino acid alanine and the alpha-ketoglutarate molecule. This process is catalyzed by the pyridoxal-5-phosphate coenzyme. ALT is present in high concentrations in the liver and is considered a sensitive marker for liver damage. It is also sometimes referred to as glutamic-alanine transaminase or glutamic-pyruvic transaminase. Overall, ALT plays a significant role in maintaining liver health and indicating potential issues with liver function.

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Measuring the Activity of Alanine Transaminase: Detection and Quantification
In order to assess the level of alanine transaminase (ALT) activity in a biological sample, it is necessary to use specific methods of detection and quantification. ALT is an enzyme that plays a key role in amino acid metabolism, and its levels can be used as a marker for liver function and various diseases.
The detection of ALT activity can be achieved through various techniques, including spectrophotometry, immunological assays, and electrophoresis. Spectrophotometry relies on the measurement of light absorption by a chromophore produced by the reaction of ALT with its substrate, and can provide a rapid and sensitive readout of enzyme activity. Immunological assays, such as enzyme-linked immunosorbent assays (ELISAs), use antibodies to specifically detect ALT in a sample. Electrophoresis separates different proteins based on their size and charge, and can distinguish ALT from other enzymes and proteins.
Once the presence of ALT activity is established, its level can be quantified by various methods, such as comparing it to a standard curve of known ALT concentrations, or calculating the rate of change of a substrate or product over time. These methods can yield accurate and precise measurements of ALT activity, which can be used to diagnose and monitor liver and other diseases.
In conclusion, detecting and quantifying alanine transaminase activity requires robust and reliable methods based on the principles of enzymology, immunology, and chemistry. By measuring ALT levels in a biological sample, clinicians and researchers can gain valuable insights into the health and pathology of the liver and other organs.

0.625 umol/ml - 20 umol/ml

Shipped and store at 4°C for 6 months.