NADP-Malate Dehydrogenase Microplate Assay Kit

NADP-malic enzyme, also known as NADP-malate dehydrogenase or malic dehydrogenase (nicotinamide adenine dinucleotide phosphate), plays a crucial role in cellular metabolism. It catalyzes the conversion of malate to pyruvate, simultaneously generating NADPH from NADP+. This essential enzyme is involved in various metabolic pathways, including the citric acid cycle and photosynthesis. Its activity is vital for maintaining redox balance in cells, as it participates in the regeneration of NADPH, a critical coenzyme in anabolic reactions and antioxidant defense systems. Given its pivotal role, NADP-malate dehydrogenase is also referred to as malate NADP dehydrogenase, NADP malate dehydrogenase, NADP-linked malate dehydrogenase, or malate dehydrogenase (NADP).

The activity of NADP-malate dehydrogenase can be detected and quantified through various methods. This enzyme plays a key role in the malate-aspartate shuttle, which is essential in the metabolism of many organisms.
One common method for detection and quantification involves measuring the rate of NADPH production from NADP+ and malate by spectrophotometry. This is accomplished by monitoring the absorbance of NADPH at 340 nm as the reaction progresses.
Another method involves using a colorimetric assay kit, which utilizes a color change to indicate the level of enzyme activity. The assay involves converting NADP+ to NADPH, which reduces a dye and produces a color change that can be measured spectrophotometrically.
Overall, the detection and quantification of NADP-malate dehydrogenase activity is important for understanding its role in cellular metabolism and for diagnosing related disorders.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.