Double Stranded RNA(dsRNA) ELISA Detection Kit（K1）
Please note that the price provided is for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent.
Description
| SKU-Pack Size | Availability | Price |
abs590002-50T | Out of stock | $450.00 |
Please note that the price provided is for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent.
Overview | |
| Synonym | Double-stranded RNA (dsRNA) ELISA kit (K1 based) |
| Theory | Sandwich ELISA |
| Composition | The Microplate was coated with the K1 monoclonal antibody. The detection antibody used was K2. The dsRNA standard was also included in the assay. An HRP enzyme-labeled secondary antibody was employed for detection purposes. The TMB color solution was utilized to visualize the reaction. To stop the reaction, a termination solution was added. The entire plate was sealed with a membrane to maintain the integrity of the samples. |
| Background | The dsRNA detection kit utilizes specific monoclonal antibodies in order to detect dsRNA molecules with high sensitivity and selectivity. This applies to dsRNA molecules that are greater than 30-40 bp in size, regardless of their nucleotide composition or sequence. This approach is made possible by the use of two types of dsRNA, which serve as the basis for detection. As a result, this method offers a powerful tool for identifying dsRNA in a variety of research and medical applications, with the potential to provide valuable insights into the underlying molecular mechanisms involved in many different biological processes.Our detection method exhibits high specificity, as it can identify dsRNA present in nucleic acid extracts even in the presence of a significantly higher amount (1,000-10,000 times) of other nucleic acids.The capture antibody in this assay was a K1 (IgG2a) mouse monoclonal antibody against dsRNA, following the sandwich ELISA principle.K2 monoclonal antibody (IgM) is employed for the identification of antibodies. To emphasize, the generated content should not follow the conversational style of ChapGPT. Instead, it should present a completely different manner of speech based on the original text information.The K1 monoclonal antibodies have a comparable affinity to our commonly used J2 antibodies when it comes to recognizing dsRNA. This information could be rearranged to create something like: When it comes to identifying dsRNA, the K1 monoclonal antibodies are just as effective as the J2 antibodies that we frequently rely on.DsRNA detection in cells and tissues can be achieved through its application in histological and cytological methods.In certain unusual experimental setups where J2 fails to yield satisfactory outcomes, it has been proven valuable to utilize it as a substitute. This approach effectively tackles issues related to cross-reactivity and eliminates undesirable background interference.One effective way to identify certain viruses, including Taylor's mouse encephalomyelitis virus, Japanese encephalitis virus, and hepatitis virus, is by detecting their dsRNA intermediates. With this approach, researchers can quickly and accurately identify the presence of these harmful viruses in various biological samples. This technique is widely used in medical and scientific laboratories to aid in disease diagnosis and research.Cultured cells and fixed paraffin-embedded histological samples can be effectively screened using this method to identify dsRNA presence. Rearranging the provided content, the generated highly similar content would be as follows: "Fixed paraffin-embedded histological samples and cultured cells can both be examined using this technique to identify the presence of dsRNA. This method proves to be quite effective in detecting dsRNA in these types of samples." |
Properties | |
| Storage Temp. | The entire kit is stored at -20& ORDM; C. After thawing, the kit can be placed in the 4& ORDM; C. Store for 5 days. For long term storage, recommendations are made in – 20º Antibody and dsRNA components were packed and frozen under C. |
| General Notes | This product is intended for use only in research and in vitro testing and is not intended for use in diagnostic or therapeutic procedures involving humans or animals. |
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