Double Stranded RNA(dsRNA) ELISA Detection Kit（J2）
Please note that the price provided above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent.
Description
| SKU-Pack Size | Availability | Price |
abs590003-50T | Out of stock | $450.00 |
Please note that the price provided above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent.
Overview | |
| Synonym | Double-stranded RNA (dsRNA) ELISA kit (J2 based) |
| Theory | Sandwich ELISA |
| Composition | The Microplate was coated with J2 monoclonal antibody. K2 detection antibody was used in conjunction with the coated Microplate. A dsRNA standard was included in the assay. An HRP enzyme-labeled secondary antibody was applied to the samples. The reaction was visualized using TMB color solution. To stop the reaction, a termination solution was added. Finally, the plate was sealed with a membrane. It is essential to note that the content above is based on the original text information provided. |
| Background | The dsRNA detection Kit is a highly sensitive and selective tool for detecting dsRNA molecules, regardless of their nucleotide composition or sequence. This is possible due to the utilization of two monoclonal antibodies that are specific to double-stranded RNA. These antibodies make it possible to detect molecules that are greater than 30-40 bp in length. The accuracy of this kit is remarkable, making it an incredibly valuable tool in the field of molecular biology. The ability to quickly and easily detect dsRNA can aid researchers in understanding the complex mechanisms of gene expression and regulation. Overall, the dsRNA detection Kit is an important resource for any scientist working in this field.The detection's specificity is remarkable as it can identify dsRNA in nucleic acid extracts even in the presence of a substantial excess of other nucleic acids, ranging from 1,000 to 10,000 times.The capture antibody used in the assay was a J2 (IgG2a) mouse monoclonal antibody against dsRNA, following the sandwich ELISA principle. Let's rearrange the content to generate a highly similar version: Utilizing the sandwich ELISA principle, the assay employed a capture antibody consisting of a J2 (IgG2a) mouse monoclonal antibody targeting dsRNA.The detection of antibodies can be facilitated by the use of monoclonal antibody K2 (IgM). This type of antibody is specifically designed and widely used for detecting the presence of other antibodies. Its reliability and specificity make it highly valuable in various research and diagnostic applications.The detection of dsRNA has been revolutionized by the use of J2 anti-DSRNA IgG2a monoclonal antibody, which has emerged as the gold standard in this field. Its precise and accurate detection mechanism has made it an indispensable asset for researchers and scientists working in this area. The J2 antibody has proved to be highly effective in detecting dsRNA, earning it a reputation for being a reliable and trustworthy tool for researchers. Its use has undoubtedly contributed immensely to the advancement of dsRNA research, and it remains a cornerstone of this field.Weber et al.'s pioneering paper introduced the application of this technique in the study of plant viruses. Although originally used for that purpose, its significance has expanded significantly.J2, a valuable tool in research, showcased in 2006 the abundant production of dsRNA by all tested plus-strand RNA viruses in infected cells. Its effectiveness has been proven across numerous systems, establishing its significance with over 200 scientific publications citing its use.Using J2 can help detect dsRNA intermediates of several viruses including hepatitis C virus, rabies virus, poliovirus, rhinovirus, chikungunya virus, dengue virus, swine fever virus, Brome Mosaic virus and many more. These viruses are commonly present in cultured cells or fixed paraffin-embedded histological samples. Therefore, J2 is a useful tool for researchers and healthcare professionals who want to detect and identify these viruses quickly and efficiently. The ability to easily identify these viruses can be beneficial in treating and preventing the spread of viral infections. |
Properties | |
| Storage Temp. | The entire kit is stored at -20& ORDM; C. After thawing, the kit can be placed in the 4& ORDM; C. Store for 5 days. For long term storage, recommendations are made in – 20º Antibody and dsRNA components were packed and frozen under C. |
| General Notes | This product is intended for use only in research and in vitro testing and is not intended for use in diagnostic or therapeutic procedures involving humans or animals. |
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