Flag-Tag Mouse Monoclonal Antibody #abs137958

abs137958-50ug

In Stock

201

abs137958-100ug

In Stock

301

Description

Looking for a top-quality Tag antibody for research purposes? Look no further than the FLAG tag Mouse mAb series. These antibodies are highly sensitive and boast an exceptional affinity for PAB, making them ideal for the detection of FLAG-tagged fusion proteins. Whether your recombinant proteins have an internal, C-terminal, or N-terminal configuration, FLAG tag antibodies are capable of detecting them all. Invest in the best with the FLAG tag Mouse mAb series.

Other names

The DDDDK epitope tag, also known as DDDK or ddk, is a widely used molecular tag in biochemistry and molecular biology research. It consists of the amino acid sequence DYKDDDDK and is derived from the enterokinase cleavage site (ECS). The ECS epitope tag, or ECS tag, is another name for this tag due to its origin from the enterokinase cleavage site.
The DYKDDDDK epitope tag is commonly utilized for the detection and purification of recombinant proteins. It can be fused to the N- or C-terminus of a target protein using genetic engineering techniques. The presence of the tag allows for easy identification and tracking of the protein of interest.
The FLAG tag antibody is widely employed for the detection and characterization of proteins containing the FLAG tag. This antibody specifically recognizes the DYKDDDDK sequence and can be utilized in various applications such as Western blotting, immunoprecipitation, and immunofluorescence.
In summary, the DDDDK epitope tag (DDDK or ddk) and the DYKDDDDK epitope tag (DYKDDDDK or DYKDDDDK tag) are interchangeable names for the same molecular tag derived from the enterokinase cleavage site (ECS). The FLAG tag antibody is commonly used to detect proteins with the FLAG tag, specifically recognizing the DYKDDDDK sequence.

Source

Mouse

Species Reactivity

All

Application

Western blotting (WB) is usually performed using dilutions of primary and secondary antibodies. The typical dilution for WB primary antibody ranges from 1:3000 to 1:10000. For immunofluorescence (IF) or immunocytochemistry (ICC), the suggested dilution for primary antibody is 1:200, while for immunoprecipitation (IP) it is also 1:200. On the other hand, when conducting ELISA using peptide antigens, the recommended dilution for antibody is much higher, typically ranging from 1:20000 to 1:40000.

Immunogen

A synthetic peptide DYKDDDDK coupled to KLH.

Properties

Concentration

1mg/ml

purification

Affinity-chromatography.

Clonality

Monoclonal Antibody

Stability & Storage

Please make sure to store it at -20 °C for a duration of one year, while avoiding any repeated freezing and thawing cycles.

Storage buffer

This solution contains Mouse IgG1 in a pH 7.4 phosphate buffered saline without Mg2+ and Ca2+ ions, with the addition of 150mM NaCl, 0.02% sodium azide, and 50% glycerol. For optimal storage, it is recommended to keep this solution at -20°C, where it remains stable for up to 12 months from the date of receipt.