Goat Anti-Mouse IgG-AlexaFluor 488 #abs20013

abs20013-100ul

In Stock

64

abs20013-500ul

In Stock

251

abs20013-1ml

In Stock

377

Description

Intact whole IgG antibodies can be isolated from antisera through immunoaffinity chromatography. These molecules possess two Fab portions that are connected by disulfide bonds and an Fc portion, rendering them divalent. The average molecular weight of these molecules is approximately 160 kDa. Due to their cost-effectiveness and compatibility with most immunodetection procedures, whole IgG forms of antibodies are commonly used.

The antibody has shown reactivity with intact mouse IgG molecules through immunoelectrophoresis and/or ELISA. It also exhibits binding to the light chains of various mouse immunoglobulins. Notably, no interaction was observed with non-immunoglobulin serum proteins. There is a possibility that this antibody might cross-react with immunoglobulins derived from other species.

Source

Goat

Specificity

IgG (H+L)

Species Reactivity

Mouse

Application

Flow cytometry, histo-/cyto-chemistry, and IF 1:100 - 1:800 are important techniques in the field of biomedical research. Histo-/cyto-chemistry involves the use of chemical stains and dyes to examine the structure and composition of cells and tissues. It allows researchers to visualize specific cellular components and identify their distribution patterns. Flow cytometry, on the other hand, is a powerful technique that enables the simultaneous analysis of multiple cellular characteristics. It uses fluorescently labeled antibodies to identify specific cell populations based on their surface markers or intracellular molecules. Finally, IF 1:100 - 1:800 refers to the dilution range typically used for immunofluorescence staining. Immunofluorescence allows researchers to detect and localize specific proteins within cells or tissues using fluorescently labeled antibodies. By combining these techniques, researchers can gain valuable insights into the molecular and cellular mechanisms underlying various biological processes.

Properties

Concentration

1mg/ml

Clonality

Polyclonal Antibody

Isotype

Whole IgG

Stability & Storage

Store at -20 °C for one year.

Storage buffer

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
：
Bovine Serum Albumin (IgG-Free, Protease-Free)15 mg/ml，0.05%Sodium Azide，50%。，。

Conjugate

AlexaFluor 488