Rabbit Anti-XBP1 Polyclonal Antibody#abs131186

abs131186-50ug

1-2 Weeks

201

abs13186-100ug

1-2 Weeks

301

Description

A crucial transcription factor is required for the growth of hepatocytes, the differentiation of plasma cells and immunoglobulin secretion, and the unfolding of the protein response (UPR). This transcription factor acts during endoplasmic reticulum stress (ER) by activating UPR target genes through its direct binding to the UPR element (UPRE). By stimulating the UPR, this transcription factor plays a vital role in maintaining cellular homeostasis, enabling the cell to cope with the stresses it encounters and promoting its survival. Its multifaceted functions make it a crucial factor in the proper functioning of various cellular processes.

Other names

XBP1 (also known as X-box binding protein 1 and tax-responsive element-binding protein 5) and XBP2 (also known as X-box binding protein 2 and tax-responsive element binding protein 5) are tax-responsive element binding proteins. They are important regulators in cellular processes and have crucial roles in the transcriptional control of genes involved in various biological functions. XBP1, also referred to as XBP1_HUMAN, is a tax-responsive element binding protein 5 that plays a vital role in cell regulation. These proteins, known as TREB5, are essential for maintaining cellular homeostasis and responding to cellular stress. They are involved in the activation of the XBP1 pathway, which is crucial for adaptive responses and survival in various cellular conditions. By rearranging the original content, it is clear that XBP1 and XBP2 are tax-responsive element-binding proteins with important roles in cellular processes and gene regulation.

Source

Rabbit

Specificity

The XBP1 Antibody is designed to identify total XBP1 at its natural levels within the body. Its ability to detect endogenous XBP1 makes it a valuable tool in research for studying this particular protein.

Predictive reaction species

Dog

Application

：
ELISA(peptide) 1:20000-1:40000, WB 1:500-1:2000, IHC 1:50-1:200。，ChapGPT，。

A synthesized peptide derived from human XBP1.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

The recommended storage condition is -20 °C for one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

The storage condition for Rabbit IgG is in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20°C and can remain stable for a period of 12 months starting from the date it is received.

Target

Background

During endoplasmic reticulum (ER) stress, this protein functions as a transcription factor by regulating the unfolded protein response (UPR). It plays important roles in embryonic development, contributing to cardiac myogenesis and hepatogenesis. Additionally, it is involved in the development of secretory tissues like the exocrine pancreas and salivary gland. Another significant function of this protein is its role in B lymphocyte differentiation into plasma cells and the production of immunoglobulins. Furthermore, it modulates the cellular response to ER stress in a PIK3R-dependent manner. It binds to the cis-acting X box found in the promoter regions of major histocompatibility complex class II genes. This protein is also crucial for VEGF-induced endothelial cell proliferation and retinal blood vessel formation during embryonic development. Moreover, it plays a role in angiogenesis in adult tissues under ischemic conditions. Interestingly, it acts as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes. Thus, it holds immense potential for the management of obesity and prevention of diabetes. Overall, this protein has diverse functions and impacts various biological processes.

Tissue specificity

Expressed in plasma cells in rheumatoid synovium (PubMed:11460154). Over-expressed in primary breast cancer and metastatic breast cancer cells (PubMed:25280941). Isoform 1 and isoform 2 are expressed at higher level in proliferating as compared to confluent quiescent endothelial cells (PubMed:19416856).

Celluar localization

Cytosol;Endoplasmic reticulum;Nucleus;

P17861