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Rabbit Anti-WWTR1 Polyclonal Antibody#abs132844

Please note that the price mentioned above is merely for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent. Western blot analysis of WWTR1 expression in Hela cell lysate,The lane on the left is treated with the antigen-specific peptide....

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Description

Catalog-specification	Delivery time	USD price
abs132844-100ug	1-2 Weeks	301.0
abs132844-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is merely for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent.

Overview
catalog	abs132844
Description	A crucial component of the Hippo signaling pathway is a transcriptional coactivator that serves as a downstream target for regulation. It plays a vital role in controlling organ size and suppressing tumor growth by limiting cell proliferation and promoting programmed cell death. The pathway's core consists of a cascade of kinases. MST1/MST2, together with its regulatory protein SAV1, phosphorylates and activates LATS1/2, which are bound to their regulatory protein MOB1. This, in turn, leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ. WWTR1 then enhances gene activation, specifically PAX8 and NKX2-1/TTF1-dependent genes. Additionally, it regulates the accumulation of SMAD proteins in the nucleus and is crucial for their interaction with the transcriptional machinery, such as the mediator complex. It also influences embryonic stem-cell self-renewal, cell proliferation, and the process of epithelial-mesenchymal transition. Please note that this content has been rearranged to maintain the accuracy of the original text but presented in a different manner as requested.
Other names	WW domain-containing transcription regulator protein 1 (WWTR1), also known as TAZ, is a transcriptional coactivator with a PDZ binding motif. It is encoded by the gene FLJ27004 and also referred to as FLJ45718 or DKFZP586I1419. In addition, it is represented by several OTTHUMP identifiers, including OTTHUMP00000215994, OTTHUMP00000215995, OTTHUMP00000215996, and OTTHUMP00000216001. WWTR1 is involved in regulating gene expression by interacting with other transcription factors. It contains a WW domain, which is responsible for protein-protein interactions, and a PDZ binding motif that enables binding to PDZ domain-containing proteins. This allows WWTR1 to function as a coactivator, enhancing the activity of transcription factors. The protein plays a crucial role in various cellular processes, including embryonic development, tissue regeneration, and stem cell maintenance. It has also been implicated in cancer progression and metastasis. Alterations in WWTR1 expression or function have been associated with different diseases and disorders. In summary, WWTR1, also known as TAZ, is a transcriptional coactivator with a PDZ binding motif. Its role in gene regulation and its involvement in various cellular processes make it an important protein with potential implications in disease development and treatment.
Source	Rabbit
Specificity	The WWTR1 antibody is capable of detecting the total endogenous levels of WWTR1 protein. To generate a highly similar content, let's rephrase the original text information. Endogenous levels of total WWTR1 can be accurately identified using the WWTR1 antibody.
Species Reactivity	Human;Mouse
Antigen	WWTR1
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	44 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
Tissue specificity	Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
Posttranslational modification	Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export.
Celluar localization	Cytosol;Nucleus;
UniPort	Q9GZV5