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Rabbit Anti-WN10B Polyclonal Antibody#abs138558

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Description

Catalog-specification	Delivery time	USD price
abs138558-100ug	1-2 Weeks	301.0
abs138558-50ug	1-2 Weeks	201.0

Overview
catalog	abs138558
Other names	The proteins Wnt-10b and Wnt-12, also known as wingless type MMTV integration site family members 10B and 12, respectively, are crucial signaling molecules involved in various biological processes. Wnt-10b has been linked to congenital hand defects and is associated with the SHFM6 gene. On the other hand, Wnt-12 is involved in embryonic development and has been shown to play a role in bone formation. These proteins share a high degree of similarity and are encoded by different genes. Understanding their functions and interactions with other molecules is important in advancing our knowledge of various diseases and developmental processes.
Source	Rabbit
Specificity	The total WN10B protein can be accurately detected using the WN10B Antibody, which is capable of recognizing the endogenous levels of this protein.
Species Reactivity	Human;Mouse
Predictive reaction species	Rabbit;Bovine;Dog;Horse;
Antigen	WN10B
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human WN10B.
MW	43 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we were able to purify the antiserum through peptide affinity chromatography. This technique allowed us to isolate the specific peptides targeted by the antiserum, resulting in a highly purified product.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality, ensure the storage temperature is -20 °C and avoid subjecting it to repeated freeze/thaw cycles. It is advisable to store it under these conditions for a year.
Storage buffer	The recommended storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate-buffered saline solution with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This storage method ensures the stability of the product for a period of 12 months from the date of receipt.
Target
Background	Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
Tissue specificity	Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
Posttranslational modification	Palmitoleylation is required for efficient binding to frizzled receptors. Depalmitoleylation leads to Wnt signaling pathway inhibition.
Celluar localization	Extracellular region or secreted;
UniPort	O00744