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Rabbit Anti-TRPV4 Polyclonal Antibody#abs138144

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Description

Catalog-specification	Delivery time	USD price
abs138144-100ug	1-2 Weeks	301.0
abs138144-50ug	1-2 Weeks	201.0

Overview
catalog	abs138144
Other names	Transient receptor potential cation channel subfamily V member 4, also known as TRPV4, is a protein encoded by the TRPV4 gene. This gene is associated with various conditions, such as BCYM3, CMT2C, and HMSN2C. It is also known by other names, including osm 9 like TRP channel 4, OTRPC 4, and SMAL. Additionally, TRPV4 is closely related to the Osm-9-like TRP channel 4 and the Osmosensitive transient receptor potential channel 4. This protein plays a role as a vanilloid receptor-like channel and is involved in the modulation of calcium and ion channels. Other names for TRPV4 include VR 4, VR-OAC, and VROAC. The protein is also associated with several disorders, including SSQTL1 and SPSMA. Overall, TRPV4 is a vital protein that is involved in many biological functions. Its association with various conditions highlights its importance in the human body and its potential as a therapeutic target. Further research is needed to fully understand the role of TRPV4 and its potential implications in disease.
Source	Rabbit
Specificity	The total TRPV4 levels can be detected using the TRPV4 Antibody to accurately measure the endogenous expression. The antibody is specifically designed to target TRPV4 and can provide reliable results in detecting the total levels of this protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Bovine;Sheep;Rabbit;
Antigen	TRPV4
Application	Here are some options: 1. For Western blotting, dilute the antibody at 1:1000 to 1:3000, while for immunofluorescence or immunocytochemistry, use a dilution of 1:100 to 1:500. If you're performing an ELISA with a peptide, the recommended antibody dilution is between 1:20000 and 1:40000. 2. Depending on your application, you may need to dilute the antibody differently. For Western blotting, try a dilution range of 1:1000 to 1:3000, while for immunofluorescence or immunocytochemistry, the recommended range is 1:100 to 1:500. If you're using the antibody for ELISA with a peptide, the dilution should be between 1:20000 and 1:40000. 3. To get the best results, make sure you're using the right dilution for your antibody. For Western blotting, start with a dilution range of 1:1000 to 1:3000, and for immunofluorescence or immunocytochemistry, use a dilution range of 1:100 to 1:500. If you're performing an ELISA with a peptide, your antibody dilution should be between 1:20000 and 1:40000. 4. It's important to optimize your antibody dilution for each application. For Western blotting, try a dilution range of 1:1000 to 1:3000, while for immunofluorescence or immunocytochemistry, the recommended range is 1:100 to 1:500. If you're using the antibody for ELISA with a peptide, the dilution should be between 1:20000 and 1:40000 to ensure accurate results.
Immunogen	A synthesized peptide derived from human TRPV4.
MW	98 kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was utilized in the purification of the antiserum through peptide affinity chromatography. The resulting product is highly purified and free from contaminants. It was a successful and efficient method for isolating the desired antibodies.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:18826956, PubMed:18695040). Also activated by heat, low pH, citrate and phorbol esters (PubMed:18826956, PubMed:18695040). Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (PubMed:12724311, PubMed:18826956). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca2+ in synoviocytes (PubMed:19759329). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (PubMed:19759329). Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage (PubMed:26249260).
Tissue specificity	Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Celluar localization	Cytoskeleton;Plasma Membrane;
UniPort	Q9HBA0