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Rabbit Anti-TOP3A Polyclonal Antibody#abs133567

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Description

Catalog-specification	Delivery time	USD price
abs133567-100ug	1-2 Weeks	301.0
abs133567-50ug	1-2 Weeks	201.0

Overview
catalog	abs133567
Other names	The gene encoding DNA topoisomerase 3 alpha, also known as DNA topoisomerase 3-alpha or TOP3A, plays a crucial role in the DNA replication process. This enzyme, often referred to as TOP3, is responsible for manipulating and altering the topological structure of DNA molecules. By efficiently unwinding, untangling, and resealing DNA strands, TOP3 ensures the proper functioning and stability of the genome. TOP3A, also called topoisomerase III alpha, is identified by its unique zinc finger, GRF-type containing 7 protein, encoded by the ZGRF7 gene. This protein is found in various organisms, including humans, and is essential for maintaining DNA integrity and preventing the accumulation of DNA damage. Studying the functions and mechanisms of DNA topoisomerase 3 alpha provides valuable insights into DNA replication, recombination, and repair processes. Its significance in genomic stability highlights the importance of understanding TOP3A's role in various cellular activities and its potential implications in diseases related to DNA damage, such as cancer. In summary, DNA topoisomerase 3 alpha, represented by TOP3A or TOP3, is a critical enzyme involved in DNA manipulation and maintenance. Its essential role in DNA replication and stability makes it a subject of interest for further research and exploration in the field of molecular biology.
Source	Rabbit
Specificity	The antibody for TOP3A is capable of detecting the complete levels of endogenous TOP3A protein present in a sample. The content can be rephrased by reorganizing the initial statement, yet it maintains the same information intact.
Species Reactivity	Human;Mouse
Antigen	TOP3A
Application	For Western blotting, a dilution range of 1:500 to 1:1000 is recommended, while for immunofluorescence or immunocytochemistry, a dilution range of 1:100 to 1:500 is optimal. When performing ELISA assays with a peptide substrate, a dilution range of 1:20000 to 1:40000 is suggested. These dilution ranges have been determined through extensive testing and are recommended for achieving reliable and accurate results. By following these guidelines, researchers can ensure that their experiments are performed under consistent conditions, allowing for more reliable and reproducible data.
Immunogen	A synthesized peptide.
MW	115 KD
Properties
Concentration	1mg/ml
purification	We employed SulfoLink™ Coupling Resin for peptide affinity chromatography in order to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, keep the store at -20 °C for a duration of one year. It is important to prevent repeated freeze/thaw cycles.
Storage buffer	The storage conditions for Rabbit IgG include storing it at -20 °C in phosphate buffered saline with a pH of 7.4. It should be maintained in a solution containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability of the IgG is guaranteed for a period of 12 months after the date of receipt. Please note that the purpose of this response is to generate a highly similar content by rearranging the information from the original text.
Target
Background	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
Tissue specificity	High expression is found in testis, heart, skeletal muscle and pancreas.
Celluar localization	Nucleus;
UniPort	Q13472