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Rabbit Anti-TOP1MT Polyclonal Antibody#abs138972

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Description

Catalog-specification	Delivery time	USD price
abs138972-100ug	1-2 Weeks	301.0
abs138972-50ug	1-2 Weeks	201.0

Overview
catalog	abs138972
Other names	The DNA topoisomerase I encoded by the gene 2900052H09Rik is an enzyme primarily found in the mitochondria. It is responsible for regulating the topological structure of DNA within the mitochondrial genome. This specific isoform, known as TOP1mt or mitochondrial topoisomerase I, plays a crucial role in maintaining the integrity and stability of the mitochondrial DNA. TOP1mt acts by introducing transient single-stranded DNA breaks, allowing the relaxation of supercoiled DNA and the resolution of DNA knots and tangles. By doing so, this enzyme prevents the accumulation of DNA damage and promotes proper DNA replication, transcription, and recombination within the mitochondria. Research on TOP1mt has revealed its significance in various cellular processes, including the maintenance of mitochondrial DNA integrity and the regulation of mitochondrial genome packaging. Mutations or dysregulation of this enzyme have been implicated in several mitochondrial disorders and age-related diseases. Understanding the functions and mechanisms of this mitochondrial topoisomerase could provide valuable insights into the fundamental processes of mitochondrial biology and the development of potential therapeutic strategies for mitochondrial-related diseases. Further investigation is necessary to unravel the precise roles and interactions of TOP1mt within the intricate network of mitochondrial genetics.
Source	Rabbit
Specificity	The TOP1MT Antibody specifically identifies the overall amount of TOP1MT present within an organism. This detection occurs at the endogenous level.
Species Reactivity	Human;Mouse;Rat
Antigen	TOP1MT
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human TOP1MT.
MW	70 kDa
Properties
Concentration	1mg/ml
purification	Through the utilization of SulfoLink™ Coupling Resin, purification of the antiserum was achieved via peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. To avoid compromising the integrity of the product, it is important to avoid exposing it to repeated cycles of freeze and thaw.
Storage buffer	Store this Rabbit IgG solution at a temperature of -20 °C in a pH 7.4 phosphate buffered saline containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. This solution is stable for a period of 12 months from the date of receipt. Please ensure proper storage conditions to maintain its integrity for extended use.
Target
Background	Mitochondrial DNA replication can cause supercoiling and torsional tension within the DNA. An enzyme known as topoisomerase helps to alleviate this tension by temporarily breaking and rejoining one strand of the DNA. This process involves introducing a single-strand break at a specific location in the DNA and forming a covalent intermediate between the enzyme and the DNA. The enzyme's catalytic tyrosine attacks the scissile phosphodiester, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand is then able to rotate around the intact phosphodiester bond on the opposing strand, thereby relieving the supercoiling tension. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate, detaching the active-site tyrosine and restoring the DNA phosphodiester backbone. Overall, topoisomerase plays an essential role in maintaining the proper structure and function of mitochondrial DNA during replication. Through its precise and highly controlled actions, this enzyme helps ensure that mitochondrial DNA remains stable and functional in cells.
Tissue specificity	Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
Celluar localization	Mitochondrion;Nucleus;
UniPort	Q969P6