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Rabbit Anti-TLR8 Polyclonal Antibody#abs135946

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Description

Catalog-specification	Delivery time	USD price
abs135946-100ug	1-2 Weeks	301.0
abs135946-50ug	1-2 Weeks	201.0

Overview
catalog	abs135946
Description	Toll-like Receptors (TLR) are a group of human receptors involved in host immunity, sharing similarities with the Toll Receptors found in Drosophila embryos, where they play a role in dorsoventral polarization during development. The TLR family is characterized by a conserved Toll homology (TH) domain, which is crucial for Toll-induced signal transductions. These receptors are type I transmembrane proteins that possess an extracellular domain with leucine-rich regions, as well as a cytoplasmic Toll/IL-1R like domain. Among the TLR family members, TLR7, TLR8, and TLR9 belong to a specific subset showing distinct expression patterns. TLR7 is mainly found in the lung, placenta, and spleen. TLR8 is expressed in the lung and peripheral blood leukocytes. TLR9, on the other hand, is predominantly found in the spleen, lymph nodes, bone marrow, and peripheral blood leukocytes. All three of these receptors activate the NF B signaling pathway, suggesting their involvement in immune responses.
Other names	The CD 288 molecule, also known as CD288 antigen, is an important Toll-like receptor protein in humans. It is encoded by the TLR8 gene and has several aliases, including MGC119599 and MGC119600. As a member of the Toll-like receptor family, TLR8 plays a crucial role in the recognition and response to invading pathogens such as viruses and bacteria. Its activation triggers a pro-inflammatory response that serves to eliminate the pathogen and promote healing. Understanding the function and regulation of TLR8 is essential for developing effective treatments for infectious diseases and other disorders that involve immune dysfunction.
Source	Rabbit
Specificity	The levels of total TLR8 in a biological sample can be detected using the TLR8 antibody. This antibody specifically recognizes and binds to endogenous TLR8 in order to measure its presence. By utilizing the TLR8 antibody, researchers can accurately quantify the amount of total TLR8 in various samples.
Species Reactivity	Human;Mouse;Rat
Antigen	TLR8
Application	The dilution ratios for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF are 1:100-500, and for ELISA (peptide) are 1:20000-1:40000. It is important to use the appropriate dilution ratio for each technique to achieve accurate and reliable results. WB requires a higher dilution compared to IHC and IF, indicating that a smaller amount of primary antibody is needed for WB. On the other hand, ELISA (peptide) requires a significantly higher dilution, suggesting that a larger amount of primary antibody is required for this technique. It is crucial to adjust the dilution ratio properly to ensure optimal signal detection and minimize background noise.
Immunogen	A synthesized peptide derived from human TLR8.
MW	115kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificity	Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
Celluar localization	Endoplasmic reticulum;Endosome;Golgi apparatus;Lysosome;Plasma Membrane;
UniPort	Q9NR97