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Rabbit Anti-SPDYA Polyclonal Antibody#abs136396

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Description

Catalog-specification	Delivery time	USD price
abs136396-100ug	1-2 Weeks	301.0
abs136396-50ug	1-2 Weeks	201.0

Dear customers,
Please note that the price mentioned above is for your reference only. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Their expertise will assist you in providing the accurate and updated pricing details.

Overview
catalog	abs136396
Description	SPDYA, also known as Speedy A, SPDY1, Ringo3, or SPY1, is a member of the speedy/ringo family and localizes to the nucleus. This 313 amino acid protein is expressed abundantly in the testis and to a lesser extent in the brain, kidney, heart, bone marrow, colon, lung, liver, spleen, and placenta. Its main function is to regulate the G1/S phase transition of the cell cycle by interacting with and activating Cdc2 p34, Cdk2, and p27. Moreover, Speedy A has been implicated in cell survival during DNA damage, indicating its role in maintaining proper cell cycle progression throughout the cell's lifespan. There are multiple isoforms of Speedy A due to alternative splicing events. The gene responsible for encoding Speedy A is found on human chromosome 2, which accounts for approximately 8% of the human genome and harbors more than 1,400 genes.
Other names	SPY/Ringo A, also known as MGC110856 or MGC57218, is a protein involved in the regulation of cell cycle progression. It is encoded by genes OTTHUMP00000082662, OTTHUMP00000082663, and OTTHUMP00000082664. In oocytes, SPY/Ringo A functions as a rapid inducer of G2/M progression, playing a crucial role in cell division and development. Other names for this protein include RINGO A, RINGO3, SPDY1, Speedy A, Speedy homolog 1 (Drosophila), Speedy homolog A, Speedy homolog A (Xenopus laevis), Speedy-1, and SPY1.
Source	Rabbit
Specificity	The total SPDYA levels can be identified using an SPDYA Antibody, which is capable of detecting the endogenous levels within the system. A rearrangement of the original content would be as follows: The SPDYA Antibody is designed to detect the endogenous levels of total SPDYA, enabling researchers to accurately measure its presence.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine
Antigen	SPDYA
Application	The recommended dilution for using the antibody in western blotting is within the range of 1:500 to 1:2000. For performing immunohistochemistry, a dilution of 1:50 to 1:200 is suggested. When using the antibody for ELISA with peptide, a dilution of 1:20000 to 1:40000 is considered to be optimal. It is important to adhere to the recommended dilution range to obtain accurate and reliable results.
Immunogen	A synthesized peptide derived from human SPDYA.
MW	36kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2.
Tissue specificity	Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase.
Celluar localization	Nucleus;
UniPort	Q5MJ70