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Rabbit Anti-RRAGA/B Polyclonal Antibody#abs134694

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Description

Catalog-specification	Delivery time	USD price
abs134694-100ug	1-2 Weeks	301.0
abs134694-50ug	1-2 Weeks	201.0

Overview
catalog	abs134694
Other names	RagA, also known as Adenovirus E3 14.7 kDa-interacting protein 1 or FIP-1, is a small protein that belongs to the Ras-related GTP-binding protein family. It plays a crucial role in cellular processes by regulating the coordination of nutrient signaling and lysosomal function. RagA interacts with other proteins, such as RagB, to form a heterodimer that controls the activation of the mechanistic target of rapamycin complex 1 (mTORC1). Through its GTP-binding activity, RagA acts as a molecular switch, allowing mTORC1 to respond to changes in nutrient availability. By rearranging the interaction partners, such as Adenovirus E3 14.7 kDa-interacting protein 1, FIP1, RagA, RagB, and Ras-related GTP-binding protein B, RagA modulates its function and influences cellular processes. Overall, understanding the role of RagA and its interacting proteins provides valuable insights into the regulation of cellular metabolism and growth.
Source	Rabbit
Specificity	The total levels of endogenous RRAGA/B can be detected using the RRAGA/B Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Xenopus;Chicken;Sheep;Bovine;Rabbit;
Antigen	RRAGA/B
Application	The dilution ratios for WB range from 1:500 to 1:1000, while the dilution ratios for IF/ICC are typically 1:100 to 1:500. On the other hand, for ELISA using a peptide, the recommended dilution ratios range from 1:20000 to 1:40000. It is important to note that these dilution ratios are based on the original text information provided.
Immunogen	A synthesized peptide.
MW	34 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. It involved a process of separating and purifying the specific peptides that were responsible for the desired target. This technique was used to achieve high specificity and purity of the antiserum, enhancing its effectiveness in targeting the intended antigen.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing to ensure optimal quality over time.
Storage buffer	To store rabbit IgG, use phosphate buffered saline at a pH of 7.4, with 150mM NaCl and 0.02% sodium azide. Add in 50% glycerol and keep it at a temperature of -20 °C. Maintaining these conditions will ensure its stability for up to 12 months from the date of receipt.
Target
Background	Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Tissue specificity	Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
Posttranslational modification	Ubiquitinated. 'Lys-68'-linked polyubiquitination of the GDP-bound inactive form of RRAGA by RNF152 is increased in response to amino acid starvation. Polyubiquitination promotes interaction with the GATOR1 complex. This does not affect RRAGA degradation.
Celluar localization	Cytosol;Endosome;Lysosome;Nucleus;
UniPort	Q7L523/Q5VZM2