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Rabbit Anti-RNF138 Polyclonal Antibody#abs141104

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Description

Catalog-specification	Delivery time	USD price
abs141104-100ug	1-2 Weeks	301.0
abs141104-50ug	1-2 Weeks	201.0

Overview
catalog	abs141104
Other names	RNF138, also known as E3 ubiquitin protein ligase RNF138 or hNARF, is a protein involved in the ubiquitination pathway. It is also referred to as HSD 4, HSD4, MGC8758, NARF, Nemo-like kinase-associated RING finger protein, NLK-associated RING finger protein, RING finger protein 138, or STRIN. RNF138 functions as an E3 ubiquitin ligase, which means it adds ubiquitin molecules to target proteins, leading to their degradation or modification. It interacts with Nemo-like kinase and plays a role in various cellular processes. The identification of RNF138 as a key player in protein degradation highlights its importance in regulating cellular functions. Understanding its mechanism and involvement in different pathways may provide insights into potential therapeutic targets for various diseases.
Source	Rabbit
Specificity	The RNF138 Antibody is capable of detecting the total endogenous levels of RNF138 protein. The generated content should maintain the same information as the original text, but with a different wording and structure to demonstrate the language model's versatility and flexibility.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine
Antigen	RNF138
Application	The recommended dilution for western blot is between 1:500 and 1:1000. For immunofluorescence and immunocytochemistry, the suggested dilution range is 1:100 to 1:500. In the case of ELISA using a peptide substrate, the recommended dilution is much higher, ranging from 1:20000 to 1:40000.
Immunogen	A synthesized peptide.
MW	28 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	-20°C。/。
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination (PubMed:26502055, PubMed:26502057). Recruited to sites of double-strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination (PubMed:26502055, PubMed:26502057). Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination (PubMed:26502055). According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP (PubMed:26502057). Together with NLK, involved in the ubiquitination and degradation of TCF/LEF (PubMed:16714285). Also exhibits auto-ubiquitination activity in combination with UBE2K (PubMed:16714285). May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway (PubMed:16714285).
Posttranslational modification	Auto-ubiquitinated.
Celluar localization	Nucleus;
UniPort	Q8WVD3