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Rabbit Anti-RNase III Drosha Polyclonal Antibody#abs138121

Please note that the price provided above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis RNase III Drosha using MCF7 whole cell lysates This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs138121-100ug	1-2 Weeks	301.0
abs138121-50ug	1-2 Weeks	201.0

Please note that the price provided above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs138121
Other names	Drosha, also known as ribonuclease III or RNase III, is a protein involved in double-stranded RNA processing. It plays a crucial role in the biogenesis of microRNAs. Drosha is a nuclear ribonuclease that specifically targets and cleaves double-stranded RNA molecules. It is also referred to as Drosha ribonuclease type III or nuclear RNase III. Another name for Drosha is Etohi2 or HSA242976. The protein Drosha, also known as p241, interacts with the transcription factor Sp1 and is considered to be a putative protein with endoribonuclease activity. Additionally, it is sometimes referred to as putative ribonuclease III or RANSE3L. Other alternative names for Drosha include RNase 3, RNase III, and RNaseN. In the context of its biological function, Drosha is responsible for processing precursor microRNAs into mature microRNAs, which play important roles in gene regulation and cellular processes.
Source	Rabbit
Specificity	The RNase III Drosha Antibody is designed to recognize total RNase III Drosha levels in the body. This antibody can effectively detect endogenous levels of the protein, allowing researchers to study its role in various biological processes. The accuracy and sensitivity of this antibody make it an important tool for investigating the function of RNase III Drosha in vivo. With the ability to identify this protein, researchers can better understand how it interacts with other molecules and contributes to the regulation of gene expression.
Species Reactivity	Human;Mouse
Antigen	RNase III Drosha
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A peptide synthesized from the human RNase III Drosha is currently under investigation. To create this peptide, scientists carefully studied the structure and function of Drosha, using that knowledge to design a highly similar synthetic molecule. By utilizing this peptide, researchers hope to better understand the intricate processes that occur within cells during gene expression and maturation. The resulting insights could ultimately lead to the development of new therapies for a variety of illnesses. While still in the early stages of research, the potential impact of this synthesized peptide is significant and exciting.
MW	159 kDa
Properties
Concentration	1mg/ml
purification	We employed SulfoLink™ Coupling Resin in peptide affinity chromatography to purify the antiserum. Alternate content: To obtain a purified antiserum, we utilized SulfoLink™ Coupling Resin in the process of peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at -20 °C for a period of one year. It is important to note that repeated freeze/thaw cycles should be avoided in order to maintain the integrity of the product. Taking these precautions will help to maximize the shelf life of the product.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This solution is stable for a period of 12 months from the date of receipt. It is important to note that the content generated should be highly similar to the original text, but rearranged in a different manner.
Target
Background	Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
Tissue specificity	Ubiquitous.
Celluar localization	Nucleus;Plasma Membrane;
UniPort	Q9NRR4