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Rabbit Anti-REV1 Polyclonal Antibody#abs132960

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Description

Catalog-specification	Delivery time	USD price
abs132960-100ug	1-2 Weeks	301.0
abs132960-50ug	1-2 Weeks	201.0

Overview
catalog	abs132960
Description	The activity of deoxycytidyl transferase is essential for DNA repair. This enzyme facilitates the transfer of a dCMP residue from dCTP to the 3'-end of a DNA primer through a reaction that relies on the presence of a template. Its role is particularly significant in the initial stage of bypassing abasic lesions, where it aids in the insertion of a nucleotide opposite the damaged site. Moreover, deoxycytidyl transferase is crucial for the normal induction of mutations caused by physical and chemical factors. Ensuring proper functioning of this enzyme is essential for maintaining the integrity and stability of the DNA molecule.
Other names	REV1 (yeast homolog) like protein, also known as Alpha integrin binding protein 80 (AIBP80), is a DNA repair protein that plays a crucial role in maintaining genomic stability. Its function is similar to that of the yeast protein REV1. The REV1 protein is involved in DNA damage response and repair mechanisms. It acts as a terminal deoxycytidyl transferase, allowing accurate DNA synthesis during the repair process. REV1 homolog is highly conserved across species, including humans. In humans, the REV1 protein is encoded by the REV1 gene. This gene is also known by several other names, such as Alpha integrin-binding protein 80, FLJ21523, MGC163283, and MGC26225. Understanding the functions of REV1 and its homologs can provide insights into DNA repair mechanisms and their implications in maintaining genome integrity.
Source	Rabbit
Specificity	The REV1 antibody has the ability to identify the total amount of REV1 present naturally in the body. This means it can detect REV1 levels that haven't been artificially altered or manipulated.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Chicken;Pig;Dog;Sheep;Bovine;Horse
Antigen	REV1
Application	The dilution ranges for WB, IHC, and ELISA assays are as follows: WB dilution ranges from 1:500 to 1:2000, IHC dilution ranges from 1:50 to 1:200, and ELISA (peptide) dilution ranges from 1:20000 to 1:40000. It is important to note the specific dilution factor for each assay to ensure accurate and reliable results. Dilutions outside of these ranges may result in suboptimal detection or excessive background noise.
Immunogen	A synthesized peptide.
MW	138 kDa
Properties
Concentration	1mg/ml
purification	We utilized SulfoLink™ Coupling Resin to perform peptide affinity chromatography in order to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
Tissue specificity	Ubiquitous.
Celluar localization	Nucleus;
UniPort	Q9UBZ9