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Rabbit Anti-RAD1 Polyclonal Antibody#abs132743

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Description

Catalog-specification	Delivery time	USD price
abs132743-50ug	1-2 Weeks	201.0
abs132743-100ug	1-2 Weeks	301.0

Overview
catalog	abs132743
Description	The 9-1-1 cell-cycle checkpoint response complex is an essential component for DNA repair. Its primary role is to be recruited to DNA damages by the replication factor C (RFC) clamp loader complex, enabling it to act as a sliding clamp platform for proteins involved in long-patch base excision repair (LP-BER). To aid in this process, the 9-1-1 complex stimulates DNA polymerase beta (POLB) activity, increasing its affinity for the 3' end of the primer-template. Within this complex, endonuclease FEN1 cleaves substrates with double, nick, or gap flaps of varying sequences and lengths, while DNA ligase I (LIG1) aids in the repair of long-patch base excision substrates. Isoform 1 of this checkpoint response complex possesses additional 3'->5' double stranded DNA exonuclease activity, which enhances its ability to play a role in critical DNA repair processes.
Other names	The cell cycle checkpoint protein RAD1 has various aliases, including HRAD1, Checkpoint control protein HRAD1, Checkpoint control protein RAD1, and DNA repair exonuclease RAD1. It is a GTP-binding protein that plays a critical role in the DNA repair process. Its homologs include DNA repair exonuclease RAD1, DNA repair exonuclease RAD1 homolog, DNA repair exonuclease REC1, and RAD1 homolog (S. pombe). Additionally, RAD1 is associated with the Ras-Related Protein Associated with Diabetes (RRAD). Its function in the DNA damage checkpoint process is similar to that of Rad1-like DNA damage checkpoint protein. EC 3.1.11.2 is also known to be a DNA repair protein RAD1. Overall, RAD1 is an important player in maintaining genome stability and preventing the accumulation of DNA damage. Its various aliases reflect its diverse functions in the cell cycle checkpoint process and its role in repairing damaged DNA.
Source	Rabbit
Specificity	The total RAD1 present at endogenous levels can be detected using the RAD1 antibody. Please rearrange the content while ensuring that the generated content is highly similar to the original text information.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	RAD1
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	32 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10846170, PubMed:10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:15314187, PubMed:15556996, PubMed:15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603). Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (PubMed:9660799).
Tissue specificity	Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
Celluar localization	Nucleus;
UniPort	O60671