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Rabbit Anti-POLR2C Polyclonal Antibody#abs136137

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Description

Catalog-specification	Delivery time	USD price
abs136137-100ug	1-2 Weeks	301.0
abs136137-50ug	1-2 Weeks	201.0

Overview
catalog	abs136137
Description	The RNA polymerase II subunit 3 gene is responsible for encoding the third largest subunit of this essential polymerase. In eukaryotes, messenger RNA synthesis relies heavily on the integrity of this polymerase. The resulting product of this gene is a heterodimer that partners with another polymerase subunit, POLR2J, to form a core subassembly unit. This gene is known to contain a cysteine rich region. Interestingly, a pseudogene has been located on chromosome 21.
Other names	The 33 kDa polypeptide of DNA-directed RNA polymerase II, also known as DNA directed RNA polymerase II subunit C or RPB3, plays a crucial role in transcription. It is encoded by the Polr2c gene and is also referred to as hRPB33 or hsRPB3. This polypeptide is an essential component of RNA polymerase II, which is responsible for transcribing DNA into RNA. It is a subunit of the polymerase complex, contributing to its stability and function. By rearranging the given information, we can create a highly similar content that maintains the essence of the original text.
Source	Rabbit
Specificity	The POLR2C Antibody is specifically designed to detect total POLR2C at endogenous levels. This means that the antibody is able to recognize the naturally occurring amounts of POLR2C within a sample. By binding to POLR2C, the antibody can be used to identify and measure the amount of this protein present in certain tissues or cells. Overall, the POLR2C Antibody is a valuable tool for researchers investigating the function and regulation of this important RNA polymerase subunit.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Horse;Bovine;
Antigen	POLR2C
Application	The recommended dilution for the Western blot is 1:500 to 1:2000, while for the immunohistochemistry it is 1:50 to 1:200. The ELISA with peptide requires a dilution of 1:20000 to 1:40000. These dilutions are based on the original content and are intended to ensure optimal results. It is important to follow the recommended dilutions to avoid any potential issues with sensitivity or specificity.
Immunogen	A synthesized peptide derived from human POLR2C.
MW	31kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	P19387