Rabbit Anti-PDX1 Polyclonal Antibody#abs136690
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Description
| Catalog-specification | Delivery time | USD price |
abs136690-100ug | 1-2 Weeks | 301.0 |
abs136690-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is for your reference only. For more detailed pricing information, it is recommended that you get in touch with our seller Vecent. It is important to emphasize that any content generated should be based on the original text, but should be crafted in a highly similar manner by rearranging the words and phrases. To avoid confusion and maintain clarity, we suggest that you do not use the ChapGPT method for creating content, but rather opt for a language model that produces text that is distinctly different from the original.
| Overview | |
catalog | abs136690 |
Description | This gene encodes a crucial transcriptional activator that regulates the expression of several important genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. This nuclear protein plays a critical role in the early development of the pancreas and is essential for glucose-dependent regulation of insulin gene expression. Mutations in this gene have been linked to pancreatic agenesis, a condition that can cause early-onset insulin-dependent diabetes mellitus (NIDDM) and maturity onset diabetes of the young type 4 (MODY4). Overall, this gene is a crucial component of the intricate network that regulates insulin production and glucose homeostasis in the body. |
| Other names | PDX1, also known as Pancreas/Duodenum homeobox protein 1 or insulin promoter factor 1 (IPF1), is a glucose-sensitive factor involved in the transcriptional regulation of insulin. PDX1 plays a critical role in the development and function of pancreatic beta cells, which are responsible for the production and secretion of insulin in response to changes in blood glucose levels. PDX1 is a homeodomain transcription factor, also known as the Islet/Duodenum homeobox 1 (IDX1) protein, that binds to specific DNA sequences within the insulin gene promoter to activate its transcription. PDX1 is crucial for the maintenance of beta cell function and survival, and mutations in the PDX1 gene can result in the development of maturity-onset diabetes of the young (MODY4). PDX1 expression is regulated by a variety of signals, including glucose, growth factors, and cytokines, and dysregulation of PDX1 expression or function can result in impaired glucose homeostasis and the development of diabetes. Therefore, PDX1 is a potential therapeutic target for the treatment of diabetes. |
| Source | Rabbit |
| Specificity | The detection of total PDX1 at its endogenous levels can be achieved using the PDX1 Antibody. By rearranging the provided information, it is emphasized that the antibody is utilized to detect the natural levels of total PDX1. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | PDX1 |
| Application | The dilution ratios for Western blotting range from 1:500 to 1:1000, while for immunohistochemistry they range from 1:50 to 1:100. When using ELISA with peptides, it is recommended to dilute the sample between 1:20000 and 1:40000. It is important to follow these dilution guidelines to ensure accurate and reliable results in these experimental techniques. |
| Immunogen | A synthetic peptide of humanPDX1. |
| MW | 31kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. |
| Tissue specificity | Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). |
| Posttranslational modification | Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. Phosphorylated by HIPK2 on Ser-268 upon glucose accumulation. This phosphorylation mediates subnuclear localization shifting. Phosphorylation by PASK may lead to translocation into the cytosol (By similarity). |
| Celluar localization | Cytosol;Nucleus; |
| UniPort | P52945 |
Western blot analysis of extracts of various celllines, using PDX1antibody|Immunohistochemistryof paraffin-embeddedhuman gastric tissue usingPDX1antibody.
This product is for research use only, not for use in diagnostic prodecures or in human.
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