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Rabbit Anti-NSF Polyclonal Antibody#abs134913

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Description

Catalog-specification	Delivery time	USD price
abs134913-100ug	1-2 Weeks	301.0
abs134913-50ug	1-2 Weeks	201.0

Overview
catalog	abs134913
Other names	N-ethylmaleimide sensitive fusion protein (NSF) is a vesicle-fusing ATPase that plays an integral role in intracellular membrane trafficking events. Also referred to as NEM-sensitive fusion protein, the NSF protein is similar in function to N-ethylmaleimide sensitive factor (NSF-like protein), and SKD2. These proteins are all involved in the disassembly of SNARE complexes, which are essential for vesicle fusion. The Vesicular fusion protein NSF is particularly important for regulating neurotransmitter release, and defects in its function have been implicated in certain types of neurological disorders. Overall, the NSF protein and its related proteins are critical for maintaining proper vesicle trafficking in cells, and disruption of their function can lead to significant cellular dysfunction.
Source	Rabbit
Specificity	The endogenous levels of total NSF can be accurately detected by the NSF Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Sheep;Dog;Horse;
Antigen	NSF
Application	To detect protein expression levels, the recommended dilution for Western blot is 1:500 to 1:1000. For immunofluorescence and immunocytochemistry, dilution ratios of 1:100 to 1:500 are suggested. If performing ELISA utilizing peptide detection, a dilution of 1:20000 to 1:40000 is optimal. Please note that these dilution ratios should be used as a starting point and optimized for individual experiments and specific conditions.
Immunogen	A synthesized peptide.
MW	75 KD
Properties
Concentration	1mg/ml
purification	Using the SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. To ensure high purity, this process was carried out meticulously.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the material, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the material to repeated cycles of freezing and thawing to prevent any potential damage.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Posttranslational modification	Phosphorylation at Ser-569 interferes with homohexamerization.
Celluar localization	Cytosol;Extracellular region or secreted;Golgi apparatus;Lysosome;Plasma Membrane;
UniPort	P46459