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Rabbit Anti-NPPA Polyclonal Antibody#abs136017

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Description

Catalog-specification	Delivery time	USD price
abs136017-100ug	1-2 Weeks	301.0
abs136017-50ug	1-2 Weeks	201.0

Overview
catalog	abs136017
Description	This gene encodes a protein that belongs to the natriuretic peptide family, which is involved in regulating extracellular fluid volume and electrolyte balance. The protein is initially synthesized as a large precursor, containing a signal peptide, that undergoes processing to release a peptide with similarities to vasoactive peptide cardiodilatin from the N-terminus. Additionally, another peptide with natriuretic-diuretic activity is released from the C-terminus. Mutations in this gene have been linked to familial type 6 atrial fibrillation. This information has been sourced from RefSeq in September 2009.
Other names	Atrial natriuretic factor, also known as ANF or ANP, is a peptide hormone that plays a crucial role in regulating blood pressure and fluid balance in the body. It is released by the atria of the heart in response to increased blood volume and stretching of the atrial walls. ANF is derived from a larger precursor protein called prepronatriodilatin, encoded by the NPPA gene. Several synonyms and abbreviations are used to refer to ANF, including ATFB6, Atriopeptin, Cardiodilatin, Cardiodilatin-related peptide, Cardionatrin, CDD ANF, CDD, CDD-ANF, CDP, Natriuretic peptide A, and PND. The main function of ANF is to promote the excretion of sodium and water by the kidneys, leading to the decrease in blood volume and blood pressure. It achieves this by inhibiting the reabsorption of sodium in the renal tubules and dilating the blood vessels. Additionally, ANF acts as a vasodilator, which means it widens the blood vessels, thereby reducing resistance to blood flow. The release of ANF is primarily regulated by the volume and pressure of blood in the heart. When blood volume increases, such as during conditions like heart failure or hypertension, the walls of the atria stretch, causing the release of ANF. ANF then acts on several target tissues, including the kidneys, blood vessels, and adrenal glands, to bring about its physiological effects. ANF has been studied extensively for its potential therapeutic applications, particularly in the treatment of cardiovascular diseases. Synthetic forms of ANF, called natriuretic peptide analogs, have been developed and tested for their ability to lower blood pressure and improve cardiac function. In conclusion, ANF, or ANP, is an important hormone that regulates blood pressure and fluid balance. It is released by the atria of the heart and acts on multiple target tissues to promote sodium and water excretion and vasodilation. Its role in cardiovascular health makes it a promising candidate for therapeutic interventions.
Source	Rabbit
Specificity	The endogenous levels of total NPPA can be detected using the NPPA Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Horse;Bovine;
Antigen	NPPA
Application	The recommended dilutions for Western Blot (WB) are 1:500-1:2000, for Immunohistochemistry (IHC) are 1:50-1:200, for Immunofluorescence/Immunocytochemistry (IF/ICC) are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. These dilutions are vital for accurate and reliable results in various experimental techniques. Adjusting the concentration appropriately ensures optimal binding of the antibodies to the target antigens, leading to successful detection and quantification of proteins or peptides. It's essential to follow these dilution guidelines to obtain consistent and reproducible data in your experiments.
Immunogen	A synthesized peptide derived from human NPPA.
MW	17kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Posttranslational modification	Cleaved by CORIN upon secretion to produce the functional hormone.Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.
Celluar localization	Extracellular region or secreted;Lysosome;Nucleus;
UniPort	P01160