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Rabbit Anti-Nogo A Polyclonal Antibody#abs138101

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Description

Catalog-specification	Delivery time	USD price
abs138101-100ug	1-2 Weeks	301.0
abs138101-50ug	1-2 Weeks	201.0

Overview
catalog	abs138101
Other names	The protein known as NogoA, or simply Nogo, is a well-studied molecule that plays a key role in regulating neurite outgrowth. Nogo was first identified as a neuroendocrine-specific protein, and subsequent research has revealed that it is a member of the reticulon family. There are several different isoforms of Nogo that have been identified, including Nogo-A, Nogo-B, and Nogo-C, which are generated by alternative splicing of the gene RTN4. One of the primary functions of Nogo is to inhibit neurite outgrowth, which is crucial for proper nervous system development and maintenance. Nogo accomplishes this by interacting with a number of different receptors, including the Nogo receptor and the paired immunoglobulin-like receptor B. Other functions of Nogo have been identified as well, including its role in regulating cellular migration and adhesion, and in the formation of myelin sheaths around axons. Research on Nogo is ongoing, and there is still much to learn about the molecule and its functions. However, the work that has been done so far has provided valuable insights into the complex regulatory mechanisms that govern nervous system development and function.
Source	Rabbit
Specificity	The presence of total Nogo A can be detected by using the Nogo A Antibody at its natural levels in the body.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Horse
Antigen	Nogo A
Application	We recommend using dilutions of 1:1000-3000 for Western blot analysis, 1:200 for immunohistochemistry, and 1:20000-1:40000 for ELISA assays with peptides. It is important to ensure that the dilutions are appropriate for the specific experiment being conducted in order to obtain accurate and reliable results. Adjustments may be necessary depending on the detection system and antigen being used. Additionally, it is recommended to optimize the dilution factors for each individual experiment to ensure maximum sensitivity and specificity.
Immunogen	A synthesized peptide derived from human Nogo A.
MW	129 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. Induces the formation and stabilization of endoplasmic reticulum (ER) tubules (PubMed:25612671, PubMed:24262037). Regulates membrane morphogenesis in the ER by promoting tubular ER production. Influences NE expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins (PubMed:26906412).
Tissue specificity	Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	Q9NQC3