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Rabbit Anti-NDC80 Polyclonal Antibody#abs136841

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Description

Catalog-specification	Delivery time	USD price
abs136841-100ug	1-2 Weeks	301.0
abs136841-50ug	1-2 Weeks	201.0

Overview
catalog	abs136841
Description	The NDC80 kinetochore complex is a critical part of the cellular machinery responsible for ensuring proper chromosome segregation during cell division. At the heart of this complex is a protein encoded by a specific gene, which is made up of two distinct domains. The first domain is responsible for binding microtubules, while the second domain forms a coiled-coil structure that facilitates interactions with other components of the complex. Together, these domains help to organize and stabilize microtubule-kinetochore interactions, ensuring that chromosomes are correctly aligned and separated during cell division. Overall, this protein is essential for the proper functioning of the NDC80 kinetochore complex, and any defects or mutations can lead to severe chromosomal abnormalities and even cancer. As such, it is crucial that we continue to study and understand the role of this protein and the complex it belongs to.
Other names	HEC, also known as Highly expressed in cancer, is a protein that shows high expression levels in cancer cells. It is characterized by the presence of leucine heptad repeats, which are repeated sequences of leucine amino acids. In humans, it is referred to as Highly expressed in cancer protein (HsHec1) or Kinetochore associated 2 (KNTC2). It is also known as Kinetochore associated protein 2 and plays a role in kinetochore function. Another name for this protein is Kinetochore protein NDC80 homolog (hsNDC80). The NDC80 homolog kinetochore complex component, also called NDC80 kinetochore complex component homolog, is the human counterpart of the NDC80 protein found in the yeast species Saccharomyces cerevisiae. It is also referred to as Retinoblastoma associated protein HEC or TID3. Overall, HEC is an important protein involved in the regulation of cell division and its high expression in cancer cells makes it a potential target for cancer therapies.
Source	Rabbit
Specificity	The NDC80 Antibody is capable of identifying total NDC80 present in the body at its natural level. Any fluctuations in the concentration of NDC80 can be detected with this antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	NDC80
Application	The recommended dilution for this antibody is WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000. These dilutions have been determined to yield optimal results based on previous testing and experimentation. It is important to note that the appropriate dilution may vary depending on the type of sample being tested and the specific experimental conditions. Therefore, it is recommended to perform a titration experiment to determine the optimal dilution for your particular application.
Immunogen	A synthesized peptide derived from human NDC80.
MW	74kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. We were able to isolate and remove impurities from the sample, leaving behind a highly purified antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).
Tissue specificity	Expression peaks in mitosis.
Posttranslational modification	Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	O14777