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Rabbit Anti-MYOD1 Polyclonal Antibody#abs137189

Description

Catalog-specification	Delivery time	USD price
abs137189-50ug	1-2 Weeks	201.0
abs137189-100ug	1-2 Weeks	301.0

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Overview
catalog	abs137189
Other names	MYF3, also known as Class C basic helix-loop-helix protein 1 or Myoblast determination protein 1, is a crucial factor involved in myogenic differentiation. It is encoded by the MYOD1 gene and is commonly referred to as MYOD or myogenin D1. The MYF3 protein plays a significant role in the determination and development of myoblasts, which are the precursor cells for muscle fibers. It acts as a transcription factor, regulating the expression of genes required for muscle formation and function. Due to its pivotal function in muscle development, MYF3 is essential for the proper differentiation of muscle cells. It is particularly involved in the formation of skeletal muscles during embryogenesis and contributes to muscle regeneration in adult organisms. The MYF3 protein belongs to the basic helix-loop-helix (bHLH) family of transcription factors. This family consists of proteins that play diverse roles in cellular processes, including cell differentiation, proliferation, and apoptosis. MYF3 specifically belongs to the Class C subgroup of bHLH proteins. In humans, the MYF3 gene is located on chromosome 11 and is expressed predominantly in skeletal muscle tissue. Its expression is tightly regulated during muscle development and is influenced by various signaling pathways and environmental cues. The functionality of MYF3 is further enhanced by its interaction with other myogenic regulatory factors, such as MYOD1. These factors form a complex network that orchestrates the sequential activation of muscle-specific genes, leading to myogenic differentiation. In conclusion, MYF3 is a critical player in the process of myogenic differentiation and is involved in the determination and development of myoblasts. Its role in muscle formation and regeneration makes it an attractive target for therapeutic interventions aiming to enhance muscle repair and treat muscle-related disorders.
Source	Rabbit
Specificity	The detection of endogenous levels of total MYOD1 can be achieved using the MYOD1 antibody. By rearranging the provided content, it is emphasized that the antibody is capable of detecting the natural levels of MYOD1 in the organism.
Species Reactivity	Human
Antigen	MYOD1
Application	ELISA (peptide) has a recommended dilution range of 1:20000-1:40000. For Western blotting, the recommended dilution range is 1:1000-3000. As for immunofluorescence (IF) and immunocytochemistry (ICC), the optimal dilution range is 1:100-1:500. It is important to note that these dilution ranges are based on the original text information and serve as guidelines for experimental purposes.
Immunogen	A synthesized peptide derived from human MYOD1.
MW	45 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. It was an effective method in removing impurities and isolating a highly pure antiserum product.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is advisable to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can adversely affect its properties. Therefore, it is recommended to store it in appropriate conditions to ensure optimal shelf life.
Storage buffer	The Rabbit IgG is stored in phosphate buffered saline at a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and remains stable for 12 months from the date of receipt. Please note that the generated content should be completely different from the original text information.
Target
Background	Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Posttranslational modification	Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).Ubiquitinated on the N-terminus; which is required for proteasomal degradation.Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
Celluar localization	Cytosol;Nucleus;
UniPort	P15172