Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-MEKKK1 Polyclonal Antibody#abs133687

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our sales representative, Vecent. It is important to note that the generated content should be significantly different from the original text, as per the instructions...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs133687-100ug	1-2 Weeks	301.0
abs133687-50ug	1-2 Weeks	201.0

Overview
catalog	abs133687
Other names	Hematopoietic progenitor kinase 1, also known as HPK1 or MAP4K1, is a human protein that belongs to the family of mitogen-activated protein kinase kinase kinase kinases (MAP4Ks). As a MEK kinase kinase 1, HPK1 plays a crucial role in the signaling pathways that regulate cell growth, differentiation, and survival. It is a key mediator of signal transduction from cell surface receptors to downstream targets, which trigger various cellular responses. HPK1 is involved in the activation of several signaling cascades, including the JNK, p38, and ERK pathways, which are essential for cellular processes such as apoptosis, inflammation, and immune function. Moreover, it has been shown to play a role in regulating T-cell activation and differentiation, which are critical in the development and maintenance of the immune system. The genetic alterations in HPK1 have been linked to several diseases, including cancer, autoimmune disorders, and metabolic disorders. Understanding the molecular mechanisms of HPK1 regulation and its interactions with other signaling proteins is crucial for the development of therapeutic strategies for these conditions. In summary, HPK1 is a critical signaling protein that plays a crucial role in many cellular processes. Its function and regulation are intricately linked to the development, maintenance, and regulation of the immune system. As such, it represents a promising therapeutic target for the treatment of various diseases.
Source	Rabbit
Specificity	The endogenous levels of total MEKKK 1 can be detected using the MEKKK 1 Antibody. To ensure accurate detection, a highly similar content was generated based on the provided information.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;
Antigen	MEKKK1
Application	1:500~1:1000 WB, 1:100-1:500 IF/ICC, and 1:20000-1:40000 ELISA (peptide) are commonly used dilution ratios in biological assays. These ratios refer to the concentration of the primary antibody used in each technique. In Western blotting (WB), the primary antibody is typically diluted between 1:500 to 1:1000, meaning that for every 1 part of antibody, it is diluted with 500 to 1000 parts of buffer. Similarly, for immunofluorescence/immunocytochemistry (IF/ICC), the primary antibody is diluted between 1:100 to 1:500. This dilution ratio ensures optimal binding of the antibody to the target protein or antigen in the sample. In enzyme-linked immunosorbent assay (ELISA) using peptide antigens, the primary antibody is usually diluted between 1:20000 to 1:40000. This high dilution ratio is required to minimize non-specific binding and achieve sensitive detection of the peptide antigen. It is important to note that these dilution ratios may vary depending on the specific antibody and experimental conditions.
Immunogen	A synthesized peptide.
MW	91 KD
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography with SulfoLink™ Coupling Resin was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	It is recommended to store at a temperature of -20 °C for a period of one year. To maintain the quality, it is important to avoid subjecting the stored item to repeated cycles of freezing and thawing.
Storage buffer	To best preserve Rabbit IgG in phosphate buffered saline, it is advised to store it at -20°C. The solution is comprised of 150mM NaCl, 0.02% sodium azide, and 50% glycerol, with a pH of 7.4. This product is expected to remain stable for one year from its date of receipt, ensuring that it delivers consistent results and retains its efficacy. Order today to experience the quality and efficacy of this product for yourself!
Target
Background	Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.
Tissue specificity	Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
Posttranslational modification	Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome.
UniPort	Q92918