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Rabbit Anti-MARK2 Polyclonal Antibody#abs133631

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Description

Catalog-specification	Delivery time	USD price
abs133631-100ug	1-2 Weeks	301.0
abs133631-50ug	1-2 Weeks	201.0

Overview
catalog	abs133631
Other names	EMK-1, also known as ELKL motif kinase 1 or ELKL motif kinase, is a serine/threonine protein kinase that belongs to the PAR1 family. It is also referred to as MAP/microtubule affinity regulating kinase 2 (MARK2) or PAR1 homolog. Its alternative names include Mark2, MARK2_HUMAN, MGC99619, Par1b, and Ser/Thr protein kinase PAR 1B. EMK-1 plays a crucial role in regulating the affinity between microtubules and MAPs (microtubule-associated proteins). Through the phosphorylation of specific target proteins, EMK-1 modulates microtubule dynamics and organization, thus influencing various cellular processes such as cell polarity, neuronal development, and cell motility. This kinase has been extensively studied in the context of neural development and cell polarity establishment. Its activity is tightly regulated and can be modulated by various extracellular signals and cellular factors. By understanding the functional mechanisms of EMK-1, researchers hope to gain insights into the fundamental processes that govern cellular organization and dynamics. In summary, EMK-1, also known as ELKL motif kinase 1 or ELKL motif kinase, is a serine/threonine protein kinase that regulates microtubule affinity through phosphorylation. It is involved in crucial cellular processes and holds promise for understanding complex cellular mechanisms.
Source	Rabbit
Specificity	The total MARK2 levels can be detected by using the MARK2 Antibody, which specifically recognizes the endogenous levels of MARK2 protein.
Species Reactivity	Human;Mouse;Rat
Antigen	MARK2
Application	For Western Blotting, the recommended dilution is between 1:500 to 1:1000. For Immunofluorescence and Immunocytochemistry, dilutions of 1:100 to 1:500 are recommended. For ELISA using a peptide, the recommended dilution is between 1:20000 to 1:40000. Please note that these dilutions are based on the original text information and should be followed accordingly. It is important to ensure accurate dilutions to achieve reliable results.
Immunogen	A synthesized peptide.
MW	85 KD
Properties
Concentration	1mg/ml
purification	A chromatography process known as peptide affinity chromatography using SulfoLink™ Coupling Resin was used to purify the antiserum. The resultant product was highly refined and effective in its application.
Clonality	Polyclonal Antibody
Stability & Storage	The recommended storage condition for this product is to keep it at -20 °C for a duration of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles.
Storage buffer	The storage condition for Rabbit IgG is in a suspension of phosphate buffered saline, with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C to maintain its stability. The product is guaranteed to be stable for a period of 12 months starting from the date of receipt.
Target
Background	Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU, and RAB11FIP2. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.
Tissue specificity	High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Posttranslational modification	Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm.
Celluar localization	Cytoskeleton;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	Q7KZI7