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Rabbit Anti-ITCH Polyclonal Antibody#abs137696

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Description

Catalog-specification	Delivery time	USD price
abs137696-100ug	1-2 Weeks	301.0
abs137696-50ug	1-2 Weeks	201.0

Overview
catalog	abs137696
Other names	Itchy E3 ubiquitin protein ligase, also known as Atrophin 1 interacting protein 4 (AIP4), is a protein involved in cellular processes such as protein degradation and regulation of gene expression. It is encoded by the ITCH gene and is present in both humans and mice. This protein functions as an E3 ubiquitin ligase, which means it helps in targeting specific proteins for degradation by attaching ubiquitin molecules to them. The AIP4 gene, also referred to as NFE2-associated polypeptide 1 (NAPP1), plays a role in the regulation of NFE2 (nuclear factor erythroid 2) activity. NFE2 is a transcription factor that regulates the expression of genes involved in the antioxidant response and protection against oxidative stress. The Itchy E3 ubiquitin protein ligase is known for its involvement in various cellular pathways, including the Notch signaling pathway, which plays a crucial role in embryonic development and tissue homeostasis. Itchy E3 ubiquitin protein ligase has been implicated in the regulation of Notch signaling by targeting certain proteins for ubiquitination and degradation. In summary, Itchy E3 ubiquitin protein ligase/AIP4 is a crucial protein involved in protein degradation and gene expression regulation. Its role in various cellular pathways highlights its importance in maintaining cellular homeostasis and proper development.
Source	Rabbit
Specificity	The ITCH Antibody has the ability to recognize total ITCH present within the body. This means that it can detect the levels of ITCH that naturally occur without any foreign intervention.
Species Reactivity	Human
Antigen	ITCH
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human ITCH.
MW	103 kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was utilized to conduct peptide affinity chromatography for the purification of the antiserum. This process yielded a high degree of purity for the final product.
Clonality	Polyclonal Antibody
Stability & Storage	For one year, the storage temperature should be maintained at -20 °C to ensure the integrity of the product. It is crucial to avoid subjecting it to repetitive freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T-helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation (By similarity). Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).
Tissue specificity	Widely expressed.
Posttranslational modification	On T-cell activation, phosphorylation by the JNK cascade on serine and threonine residues surrounding the PRR domain accelerates the ubiquitination and degradation of JUN and JUNB. The increased ITCH catalytic activity due to phosphorylation by JNK1 may occur due to a conformational change disrupting the interaction between the PRR/WW motifs domain and the HECT domain and, thus exposing the HECT domain (By similarity). Phosphorylation by FYN reduces interaction with JUNB and negatively controls JUN ubiquitination and degradation.Ubiquitinated; autopolyubiquitination with 'Lys-63' linkages which does not lead to protein degradation.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	Q96J02