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Rabbit Anti-ILF3 Polyclonal Antibody#abs136485

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Description

Catalog-specification	Delivery time	USD price
abs136485-100ug	1-2 Weeks	301.0
abs136485-50ug	1-2 Weeks	201.0

Overview
catalog	abs136485
Description	The product of this gene is a binding protein that interacts with various molecules, including double-stranded RNA (dsRNA), small noncoding RNAs, and mRNAs. These interactions play a crucial role in regulating gene expression and stabilizing mRNAs. Initially identified as a component of the nuclear factor of activated T-cells (NFAT), this protein serves as a subunit of NFAT, which is essential for T-cell expression of interleukin 2. NFAT consists of two proteins, one weighing 45 kDa and the other 90 kDa, with the larger being the product of this gene. These proteins have been found to influence the movement of nuclear mRNA to the cytoplasm. Suppressing the expression of NF45 or NF90 protein has been shown to decelerate cell growth, potentially by impeding mRNA stabilization. Conversely, another version of this gene, known as NF110, is primarily localized in the nucleus and has only minor effects on cell growth when its levels are reduced. The process of alternative splicing produces multiple transcript variants that encode distinct isoforms of the protein. This information is based on data provided by the Reference Sequence database in November 2008.
Other names	DRBF, also known as double-stranded RNA-binding protein 76 or double-stranded RNA-binding protein 76, is a critical protein involved in various cellular processes. It is also referred to as ILF3 or interleukin enhancer binding factor 3. Another name for this protein is M-phase phosphoprotein 4 or MPHOSPH4. It is sometimes called NF AT 90 or NF-AT-90, NF110, or NF90. It is associated with the regulation of gene expression and translation control. Moreover, it is involved in the recognition and binding of double-stranded RNA molecules. NFAR, which stands for nuclear factor associated with dsRNA, is another term used to describe this protein. It is also known as nuclear factor of activated T cells 90 kDa or nuclear factor of activated T-cells 90 kDa. Additionally, this protein is sometimes called TCP80 or translational control protein 80.
Source	Rabbit
Specificity	The endogenous levels of total ILF3 can be detected using the ILF3 Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Chicken;Sheep;Dog;Bovine
Antigen	ILF3
Application	The dilutions for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human ILF3.
MW	95kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552).
Tissue specificity	Ubiquitous.
Posttranslational modification	Phosphorylated at Thr-188 and Thr-315 by PKR in response to certain RNA viruses. This phosphorylation results in the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3 where it can bind to viral RNAs and impede viral replication.Methylated by protein arginine N-methyltransferase 1.
Celluar localization	Mitochondrion;Nucleus;
UniPort	Q12906