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Rabbit Anti-IL15RA Polyclonal Antibody#abs135009

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Description

Catalog-specification	Delivery time	USD price
abs135009-100ug	1-2 Weeks	301.0
abs135009-50ug	1-2 Weeks	201.0

Overview
catalog	abs135009
Other names	The IL-15 receptor subunit alpha, also known as IL-15R-alpha, plays a crucial role in the immune system. This protein is encoded by the Il15ra gene and is a component of the receptor complex that binds to IL-15, a cytokine that regulates the growth and activation of various immune cells. Other names for IL-15R-alpha include CD215, Interleukin-15 receptor subunit alpha, and sIL-15R-alpha. IL-15R-alpha is expressed on the surface of various immune cells, including natural killer cells and memory CD8+ T cells. It forms a heterotrimeric receptor complex with the IL-2 receptor beta and gamma chains, allowing for the transmission of IL-15 signals into the cell. IL-15R-alpha can also be shed from the surface of cells and circulate in the blood as a soluble form, known as sIL-15R-alpha. The soluble form of IL-15R-alpha, in particular, has been found to regulate immune responses in a variety of ways. It can act as a decoy receptor, binding to IL-15 and preventing it from binding to its membrane-bound receptor. This can reduce the availability of IL-15 to immune cells, leading to immune suppression. However, sIL-15R-alpha can also act as an agonist, inducing signaling pathways in certain immune cells and promoting their activation and proliferation. Overall, IL-15R-alpha is a complex and versatile protein that plays an important role in immune regulation. Its expression and function are tightly controlled, and its dysregulation has been implicated in various immune-related disorders. Further research into this protein and its roles in the immune system may lead to new therapies for these diseases.
Source	Rabbit
Specificity	The total IL15RA levels present in the body can be detected using IL15RA Antibody. It specifically targets and identifies the endogenous levels of IL15RA.
Species Reactivity	Human;Rat
Antigen	IL15RA
Application	For Western Blotting, the recommended dilution range is 1:500 to 1:1000. For Immunofluorescence or Immunocytochemistry, a dilution of 1:100 to 1:500 is suggested. For ELISA with peptide detection, the optimal dilution range is 1:20000 to 1:40000. These dilutions have been determined to provide the most accurate and reliable results for each assay. It is important to follow the recommended dilution guidelines to ensure the best performance of the antibody in the desired application.
Immunogen	A synthesized peptide.
MW	32 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To achieve this, the resin was coupled with specific peptides that bound to the antibodies in the antiserum. The target antibodies were then extracted, leaving behind impurities such as excess proteins and cells. This process resulted in a highly purified antiserum primed for further usage in various diagnostic and research applications.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is important to keep it stored at a temperature of -20°C for up to twelve months. It is recommended to avoid subjecting the product to repeated freeze/thaw cycles as this may negatively impact its effectiveness.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it is stored at a temperature of -20 °C in a solution of phosphate buffered saline with a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it in this manner for a maximum of 12 months starting from the date of receipt.
Target
Background	High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves SYK.
Tissue specificity	Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
Posttranslational modification	A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.Phosphorylated by activated SYK.N-glycosylated and O-glycosylated.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Nucleus;Plasma Membrane;
UniPort	Q13261