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Rabbit Anti-H3F3A Polyclonal Antibody#abs137365

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Description

Catalog-specification	Delivery time	USD price
abs137365-100ug	1-2 Weeks	301.0
abs137365-50ug	1-2 Weeks	201.0

Overview
catalog	abs137365
Other names	The H3 histone family has three members, including H3 histone family 3A, H3 histone family 3B, and H3 histone family 3B (H3.3B). These members are also known as H3.3, H3.3A, and H3.3B, respectively. Additionally, they are referred to as H3F3, H3F3A, and H3f3b in some cases. Other names for H3 histone family 3B include Histone H3.3Q, MGC87782, and MGC87783. Overall, these histones are important components of chromatin that play critical roles in regulating gene expression, DNA repair, and other cellular processes. Although they are highly similar in sequence, each member of the H3 histone family exhibits unique properties that contribute to their functional diversity.
Source	Rabbit
Specificity	The detection of total H3F3A levels can be achieved using the H3F3A Antibody, which is capable of identifying endogenous levels of this protein. To clarify, the antibody allows researchers to measure the overall presence of H3F3A within a sample.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Xenopus;Chicken;Bovine;Zebrafish
Antigen	H3F3A
Application	The recommended dilution range for WB is from 1:1000 to 3000, while for IHC it is 1:200. For ELISA using peptide, the recommended dilution range is from 1:20000 to 1:40000. It is important to follow these dilution guidelines to ensure accurate and reproducible results. By carefully adjusting the dilution, researchers can obtain optimal signal-to-noise ratios and avoid background noise. Additionally, it is always recommended to validate the results using appropriate positive and negative controls. Overall, these dilution ranges are based on empirical observations and are tailored to each specific assay, ensuring reliable and sensitive detection of target proteins.
Immunogen	A synthesized peptide derived from human H3F3A.
MW	19 kDa
Properties
Concentration	1mg/ml
purification	The purification process involved utilizing SulfoLink™ Coupling Resin for peptide affinity chromatography to obtain a purified form of the antiserum. Please note that the generated content is based on the original text information but rearranged to provide a highly similar version.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of the product, it is recommended to store it at a temperature of -20 °C for a maximum of one year. It is important to note that the product should not be subjected to multiple freeze/thaw cycles, as this can cause damage to the composition. To maintain the integrity of the product, it is essential to adhere to the recommended storage guidelines.
Storage buffer	The rabbit IgG provided is suspended in a solution of phosphate buffered saline, with a pH of 7.4, 150mM NaCl concentration, and a trace amount of sodium azide. Additionally, the solution includes 50% glycerol, which aids in storage at a freezing temperature of -20°C. The shelf life of this product is approximately one year from the date of receipt, ensuring a stable and reliable supply for your research needs.
Target
Background	Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Tissue specificity	Expressed throughout the cell cycle independently of DNA synthesis.
Posttranslational modification	Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin. Phosphorylation on Ser-32 (H3S31ph) is specific to regions bordering centromeres in metaphase chromosomes.Ubiquitinated. Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity).Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (PubMed:22483618).Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	P84243