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Rabbit Anti-GCLM Polyclonal Antibody#abs136780

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Description

Catalog-specification	Delivery time	USD price
abs136780-50ug	1-2 Weeks	201.0
abs136780-100ug	1-2 Weeks	301.0

Overview
catalog	abs136780
Description	Gamma-glutamylcysteine synthetase, also called glutamate-cysteine ligase, serves as the initial limiting enzyme in the synthesis of glutathione. Composed of a heavy catalytic subunit and a light regulatory subunit, this enzyme plays a crucial role. Deficiency in gamma-glutamylcysteine synthetase has been linked to certain forms of hemolytic anemia.
Other names	The Gamma ECS regulatory subunit, also known as the Gamma-ECS regulatory subunit or the Gamma-glutamylcysteine synthetase regulatory subunit, is an important component of the enzyme glutamate cysteine ligase. It is encoded by the GCLM gene and is also referred to as GCS light chain or GLCLR. This regulatory subunit plays a crucial role in the synthesis of glutathione, a powerful antioxidant that helps protect cells from oxidative stress. The Gamma ECS regulatory subunit is involved in the catalytic activity of glutamate cysteine ligase, which is responsible for the first and rate-limiting step in glutathione biosynthesis. It acts as a modifier subunit, regulating the activity of the enzyme complex and influencing its overall function. Alterations in the expression or function of the Gamma ECS regulatory subunit can have significant impacts on cellular antioxidant capacity and redox homeostasis. Dysregulation of glutathione synthesis has been associated with various pathological conditions, including neurodegenerative disorders, cancer, and cardiovascular diseases. Understanding the role of the Gamma ECS regulatory subunit in glutathione metabolism provides valuable insights into cellular antioxidant defense mechanisms and may have implications for the development of therapeutic strategies targeting oxidative stress-related diseases. Further research is warranted to unravel the precise molecular mechanisms underlying the regulation of glutamate cysteine ligase and its regulatory subunit.
Source	Rabbit
Specificity	GCLM Antibody is capable of identifying the natural levels of total GCLM within an organism. Take note that the following content has been rearranged to maintain the original information of the text.
Species Reactivity	Human;Mouse;Rat;Pig
Predictive reaction species	Pig;Bovine;Rabbit
Antigen	GCLM
Application	IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000. These are the recommended dilutions for performing immunohistochemistry (IHC), Western blot (WB), and enzyme-linked immunosorbent assay (ELISA) using peptide antigens. Dilutions of 1:50-1:200 for IHC, 1:500-1:2000 for WB, and 1:20000-1:40000 for ELISA are suggested to achieve optimal results.
Immunogen	A synthesized peptide derived from human GCLM.
MW	30kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography. The resulting product was highly purified and free from any impurities.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Tissue specificity	In all tissues examined. Highest levels in skeletal muscle.
Celluar localization	Cytosol;
UniPort	P48507