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Rabbit Anti-GCK Polyclonal Antibody#abs138069

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Description

Catalog-specification	Delivery time	USD price
abs130069-50ug	1-2 Weeks	201.0
abs130069-100ug	1-2 Weeks	301.0

Overview
catalog	abs130069
Description	FOS is a bZIP family transcription factor that can become proto-oncogenic. It has the ability to dimerize with JUN family proteins, which results in the formation of the transcription factor complex known as AP-1. FOS plays a critical role in regulating cell proliferation, transformation, and differentiation. Interestingly, research has also linked the expression of FOS to apoptotic cell death under certain conditions. The expression of this protein increases in response to various stimuli such as stress, cytokines, growth factors, polypeptide hormones, and neurotransmitters, as well as cell injury in general.
Other names	AP-1, also known as activator protein 1, is a transcription factor composed of FOS and JUN proteins. FOS, or proto-oncogene c-Fos, is a cellular oncogene that was originally identified as the viral oncogene FBJ murine osteosarcoma viral (v-fos) homolog. FBJ murine osteosarcoma viral oncogene homolog, or FOS, plays a crucial role in regulating gene expression in various biological processes, including cell growth, differentiation, and apoptosis. The protein is encoded by the FOS gene and is capable of forming homodimers or heterodimers with other proteins like JUN. These dimers bind to DNA in the promoter region of target genes and activate their transcription by recruiting transcriptional coactivators or remodeling chromatin. Dysfunction of FOS has been implicated in numerous diseases, such as cancer, osteoporosis, and inflammatory disorders. G0/G1 switch regulatory protein 7, or G0S7, is a protein that can inhibit the activity of FOS in some cell types. Overall, the regulation and interaction of FOS and other AP-1 proteins are crucial for maintaining cellular homeostasis and proper physiological functions.
Source	Rabbit
Specificity	The Fos antibody has the ability to recognize and measure the amount of total Fos that is naturally present within a cell or tissue sample. This means that it can accurately detect the endogenous levels of Fos, without interference from any external sources or manipulation.
Species Reactivity	Human;Mouse;Rat
Antigen	Fos
Application	For Western blotting, the recommended dilution range for this antibody is 1:500 to 1:3000. When using it for immunohistochemistry, it is suggested to dilute the antibody within a range of 1:50 to 1:200. For immunofluorescence and immunocytochemistry, you should dilute the antibody between 1:100 and 1:500. Finally, for ELISA peptide detection, the antibody dilution should be 1:20000 to 1:40000. Please make sure to adhere to the recommended dilution ranges for optimal results.
Immunogen	A synthesized peptide derived from human Fos.
MW	50kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Tissue specificity	Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.
Posttranslational modification	Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.
Celluar localization	Cytosol;Endoplasmic reticulum;Nucleus;
UniPort	P01100