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Rabbit Anti-EPHX1 Polyclonal Antibody#abs136319

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Description

Catalog-specification	Delivery time	USD price
abs136319-100ug	1-2 Weeks	301.0
abs136319-50ug	1-2 Weeks	201.0

Overview
catalog	abs136319
Description	Epoxide hydrolase is a crucial enzyme that plays a crucial role in the biotransformation process by converting epoxides, which are produced during the breakdown of aromatic compounds, into trans-dihydrodiols. These compounds can then be conjugated and eliminated from the body. Epoxide hydrolase is involved in both the activation and detoxification of epoxides, and any mutations in this gene can lead to a variety of conditions such as preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. There are also several alternative spliced transcript variants encoding the same protein found for this gene. Overall, epoxide hydrolase is a critical enzyme that helps to maintain the proper functioning of the body's detoxification system.
Other names	EPHX 1, also known as EPHX or Ephx1, is a gene that codes for the enzyme epoxide hydrolase 1. This enzyme is also referred to as Epoxide Hydratase or Epoxide Hydroxylase 1 and plays a critical role in the metabolism and detoxification of xenobiotic compounds in the human body. The microsomal version of Epoxide hydrolase 1 is particularly important in the metabolism of epoxides, which are reactive intermediates generated during the metabolism of a variety of chemicals. The enzyme catalyzes the hydrolysis of epoxides, converting them into relatively inert diols. Deficiencies in EPHX 1 have been linked to an increased risk of several diseases, including cancer and cardiovascular disease. Proper regulation and functioning of EPHX 1 is crucial for maintaining optimal health and wellbeing.
Source	Rabbit
Specificity	The EPHX1 Antibody is capable of detecting total EPHX1 at its natural levels within the body. A similar wording could be, "Total levels of EPHX1 can be identified with the help of the EPHX1 Antibody, which detects the endogenous expression of the protein."
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Zebrafish;Horse;Chicken
Antigen	EPHX1
Application	The recommended dilution factors for using the antibody in various applications are as follows: For Western blotting, a dilution of 1:500 to 1:2000 is recommended, for immunohistochemistry a dilution of 1:50 to 1:200 is suggested, and for ELISA using peptide substrates, it is recommended to use a dilution between 1:20000 and 1:40000. Ensure that you follow the provided instructions carefully while using the antibody to obtain optimal results in your experiments.
Immunogen	A synthesized peptide derived from human EPHX1.
MW	53kDa
Properties
Concentration	1mg/ml
purification	Through the use of SulfoLink™ Coupling Resin, peptide affinity chromatography was employed in the purification of the antiserum. In this process, the resin was coupled with specific peptides to selectively bind and isolate the desired molecules. The resulting purified antiserum was effective and of high quality for its intended use.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
Tissue specificity	Found in liver.
Celluar localization	Endoplasmic reticulum;Plasma Membrane;
UniPort	P07099