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Rabbit Anti-EME1 Polyclonal Antibody#abs134973

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Description

Catalog-specification	Delivery time	USD price
abs134973-100ug	1-2 Weeks	301.0
abs134973-50ug	1-2 Weeks	201.0

Overview
catalog	abs134973
Other names	The Crossover junction endonuclease EME1, also known as Essential Meiotic Endonuclease 1 Homolog 1 (S. pombe), is an enzyme that plays a crucial role in meiotic recombination. It is encoded by the EME1 gene, which codes for a protein that is highly conserved across various species. EME1 is a type of endonuclease that cleaves DNA at certain points, facilitating the exchange of genetic material between homologous chromosomes during meiosis. It is essential for the accurate segregation of chromosomes during the first meiotic division. Mutations in the EME1 gene have been linked to meiotic defects and infertility in both males and females. In addition to its role in meiosis, EME1 has also been implicated in DNA damage repair. It forms a complex with another protein, MMS4, and together they play a critical role in the repair of DNA double-strand breaks. Mutations in the EME1-MMS4 complex have been associated with increased sensitivity to certain DNA-damaging agents, such as ionizing radiation and certain chemotherapeutic drugs. Overall, EME1 is a crucial enzyme for maintaining genomic stability and ensuring proper meiotic recombination and DNA repair. Its importance is underscored by the fact that it is highly conserved across species, from yeast to humans.
Source	Rabbit
Specificity	The total levels of endogenous EME1 can be effectively detected using the EME1 Antibody. Rearranging the original content, we find that this antibody is specifically designed to identify and measure the endogenous expression of EME1. By targeting the total EME1 protein, this antibody enables researchers to accurately quantify its presence in cells or tissues. This information is vital for studying the role and regulation of EME1 in various biological processes. With the EME1 Antibody, researchers can gain insights into the functions and mechanisms of EME1, opening new avenues for scientific exploration.
Species Reactivity	Human
Predictive reaction species	Rabbit
Antigen	EME1
Application	The dilution range for Western Blot is 1:500~1:1000, for Immunofluorescence/Immunocytochemistry it is 1:100-1:500, and for ELISA with peptide it is 1:20000-1:40000. Feel free to rearrange the presented information to create a highly similar content.
Immunogen	A synthesized peptide.
MW	65 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. To ensure the highest quality of the antiserum, this process was carefully selected to remove any unwanted impurities. With this targeted method of purification, the resulting antiserum is highly effective at targeting specific pathogens and has been shown to be a reliable tool in research and clinical applications.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended that this product be kept at a temperature of -20 °C for a period of one year. It is important to avoid allowing the product to go through multiple freeze/thaw cycles, as this can potentially damage the quality of the product. Therefore, it is important to handle and store this product with care to maintain its integrity.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
Celluar localization	Nucleus;
UniPort	Q96AY2