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Rabbit Anti-EIF2AK1 Polyclonal Antibody#abs141130

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Description

Catalog-specification	Delivery time	USD price
abs141130-100ug	1-2 Weeks	301.0
abs141130-50ug	1-2 Weeks	201.0

Overview
catalog	abs141130
Other names	EIF2AK1, also known as HRI or Heme-controlled repressor, is a crucial enzyme that belongs to the family of serine/threonine protein kinases. This enzyme is responsible for regulating the initiation of protein synthesis in response to various environmental cues, such as heme availability. It is also involved in mediating cellular stress responses, such as oxidative stress, hypoxia, and nutrient deprivation. EIF2AK1 is a highly conserved protein that plays a crucial role in the cellular signaling pathway of stress response. Dysfunction of this protein can lead to various diseases, including cancer, neurodegeneration, and metabolic disorders. Thus, understanding the cellular mechanisms underlying the function of EIF2AK1 is crucial for developing potential therapeutic strategies for various human diseases.
Source	Rabbit
Specificity	The EIF2AK1 Antibody is capable of detecting total EIF2AK1 levels present within the body. This antibody is designed to specifically target endogenous levels of EIF2AK1.
Species Reactivity	Human;Mouse
Antigen	EIF2AK1
Application	WB 1:500~1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 ：，（Western Blot），1:5001:1000。（Immunofluorescence）（Immunocytochemistry），1:1001:500。ELISA，（Enzyme-Linked Immunosorbent Assay），1:200001:40000。，。
Immunogen	A synthesized peptide.
MW	71 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, peptide affinity chromatography was employed to purify the antiserum. A highly similar content can be generated by rearranging the sentence structure and maintaining the key information provided in the original text.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to multiple freeze and thaw cycles, as this may compromise its efficacy. Proper storage of the product will ensure its potency and efficacy for its intended use. Please handle the product with care to guarantee its maximum effectiveness.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).
Tissue specificity	Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level).
Posttranslational modification	Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations (By similarity).
UniPort	Q9BQI3