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Rabbit Anti-DPYD Polyclonal Antibody#abs136027

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Description

Catalog-specification	Delivery time	USD price
abs136027-100ug	1-2 Weeks	301.0
abs136027-50ug	1-2 Weeks	201.0

Overview
catalog	abs136027
Description	DPD, also known as DPYD, is responsible for the initial and rate-limiting step in the break down of uracil and thymidine, as well as the chemotherapeutic drug 5-fluorouracil (5-FU) and its derivatives. Mutations in the DPYD gene can lead to DPYD deficiency, causing problems in pyrimidine metabolism and potentially dangerous side effects in cancer patients who are treated with 5-FU. Researchers are exploring whether variations in the DPYD gene and protein expression can predict 5-FU toxicity. Recent studies have identified several genes that confer resistance to 5-FU in human hepatocellular carcinoma (HCC). AEG-1 is one such gene, which is highly expressed in HCC and increases the expression of DPYD. DPYD is expressed more abundantly in HCC than in normal liver tissue, providing a possible mechanism for 5-FU resistance in this setting.
Other names	Dihydropyrimidine dehydrogenase [NADP(+)], also known as DHP or DPYD, plays a crucial role in catalyzing the oxidation of dihydropyrimidines to their corresponding uracil derivatives. This enzyme is essential for the normal metabolism of pyrimidine bases found in DNA and RNA. Dihydrothymine dehydrogenase and dihydrouracil dehydrogenase are alternative names for DHP, highlighting its involvement in the catabolism of thymine and uracil, respectively. DPYD_HUMAN, MGC132008, and MGC70799 are gene symbols and identifiers associated with the human dihydropyrimidine dehydrogenase gene. OTTHUMP00000058954 is an accession number for a specific sequence of the DPYD gene. DHPDHase, DHP, and DPYD are abbreviations commonly used to refer to dihydropyrimidine dehydrogenase. Its activity relies on the cofactor NADP(+) (nicotinamide adenine dinucleotide phosphate), which serves as an electron acceptor during the oxidation reaction. Please note that the above content has been rearranged to provide a similar description while maintaining the original information.
Source	Rabbit
Specificity	The DPYD Antibody is designed to recognize total DPYD present in the body. Its main function is to detect the endogenous levels of DPYD and provide accurate results. The antibody is a valuable tool in research and diagnosis as it helps in detecting any anomalies related to DPYD levels. Overall, the DPYD Antibody plays an important role in understanding the functioning of the DPYD protein in the body.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	DPYD
Application	For Western blotting, a dilution range of 1:500 to 1:2000 is recommended. Immunohistochemistry studies should use a dilution range of 1:50 to 1:200. For immunofluorescence and immunocytochemistry studies, a dilution range of 1:100 to 1:500 is optimal. When performing ELISA using a peptide substrate, a dilution range of 1:20,000 to 1:40,000 is suggested. It is important to note that these dilutions may need to be adjusted based on the specific application and experimental conditions.
Immunogen	A synthesized peptide derived from human DPYD.
MW	110kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.
Tissue specificity	Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.
Celluar localization	Cytosol;
UniPort	Q12882